Morphological, molecular, and chromosomal discrimination of cryptic Anopheles (Nyssorhynchus) (Diptera: Culicidae) from South America.
Morphological, molecular, and chromosomal discrimination of cryptic Anopheles (Nyssorhynchus) (Diptera: Culicidae) from South America.
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DOI：
10.1093/jmedent/35.5.830
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发表时间：
1998-09
影响因子：
2.1
通讯作者：
L. Lounibos;R. Wilkerson;Jan E. Conn;L. Hribar;Gary N. Fritz;J. Danoff-Burg
中科院分区：
文献类型：
--
作者：
L. Lounibos;R. Wilkerson;Jan E. Conn;L. Hribar;Gary N. Fritz;J. Danoff-Burg
根据男性生殖器的相似性，Peyton(1993)将来自哥伦比亚、厄瓜多尔、秘鲁和玻利维亚东部安第斯山麓的疟疾媒介按蚊确定为An的初级异名。Dunhami Causey，当时在亚马孙巴西的一个地方出名。经过分子、染色体和形态特征的鉴定，我们在此得出结论，这两个分类群是特别不同的，并去除了一个。Tinkae与An的同义词。敦哈米。这两个物种的卵很容易通过前冠、长长的漂浮物和封闭的甲板来区分，这些只出现在一个物种中。特林卡。多线虫幼虫的X染色体可分为R臂和L臂。Dunhami，但不是在一个。特林卡。根据18个100%分辨率的随机扩增多态性DNA引物的带型得分绘制的表型图。Dunhami，An.Tinkae、印度按蚊Anopheles nuneztovari Gabaldón和达氏按蚊Anopheles darlingi Root.在rDNA的ITS2区，25%的碱基可区分An。天南星科An.Dunhami和21%来自相关的An。Nuneztovari；这3种雄性的附腺大小明显不同。同工酶初步筛选表明，11个基因座中有3个基因座可用于分离An。天南星科An.敦哈米。结果表明，An.Dunhami与An的亲缘关系更密切。Nuneztovari比An.并说明了采用多学科方法研究蚊子系统学的优点。
Based on similarity of male genitalia, the malaria vector Anopheles trinkae Faran from the eastern Andean piedmont of Colombia, Ecuador, Peru, and Bolivia was determined by Peyton (1993) to be a junior synonym of An. dunhami Causey, then known from a single locality in Amazonian Brazil. Following an appraisal of molecular, chromosomal, and morphological characters, we conclude herein that the 2 taxa are specifically distinct and remove An. trinkae from synonymy with An. dunhami. Eggs of the 2 species are distinguished easily by the anterior crown, long floats, and closed deck that occur only in An. trinkae. The X chromosome of larval polytenes is divisible into R and L arms in An. dunhami, but not in An. trinkae. A phenogram based on banding pattern scores from 18 random amplified polymorphic DNA primers separated with 100% resolution An. dunhami, An. trinkae, Anopheles nuneztovari Gabaldón and Anopheles darlingi Root. In the ITS2 region of rDNA, 25% of base sites distinguished An. trinkae from An. dunhami and 21% from the related An. nuneztovari; males of these 3 species had accessory glands of significantly different sizes. Preliminary isoenzyme screening indicated that 3 of 11 loci were diagnostic for separating An. trinkae from An. dunhami. The results indicate that An. dunhami is related more closely to An. nuneztovari than to An. trinkae and illustrate the merits of a multidisciplinary approach to mosquito systematics.