Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development
Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development
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DOI：
10.1074/mcp.m110.005231
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发表时间：
2011-03-01
影响因子：
7
通讯作者：
Kussmann, Martin
中科院分区：
文献类型：
--
作者：
Hansson, Jenny;Panchaud, Alexandre;Kussmann, Martin
出生后肠道发育是一个非常动态的过程，其特征是大量的形态变化，与断奶后从富含脂肪（牛奶）的饮食到富含碳水化合物的饮食的营养变化的功能适应相一致。到目前为止，对肠道发育的时间分辨研究仅限于在转录水平上进行研究，或者一次只研究单个或少数蛋白质。本研究采用无标记蛋白质组学方法，研究了哺乳早期（1-7日龄）、哺乳中期（7-14日龄）和断奶期（14-35日龄）小鼠空肠原代肠上皮细胞蛋白质组学的变化。我们显示在肠道发育过程中的差异表达的520种蛋白质和蛋白质组的显着变化，在中期哺乳期和断奶。蛋白质参与几个代谢过程中发现差异表达沿着发展。发现糖酵解酶的时间表达谱与断奶时碳水化合物摄取的增加相关，而参与脂肪酸代谢和乳糖代谢的蛋白质的丰度变化表明断奶时营养变化的非饮食驱动准备。此外，我们报告了在新生儿获得被动免疫中起重要作用的蛋白质的发育丰度变化。此外，几种蛋白质的不同亚型进行了定量，这可能有助于更好地了解特定亚型在小肠中的作用。总之，我们提供了第一个，时间分辨的蛋白质组谱肠上皮细胞沿着出生后肠道发育。Molecular & Cellular Proteomics 10：10.1074/mcp. M110.005231，1-12，2011.
Postnatal intestinal development is a very dynamic process characterized by substantial morphological changes that coincide with functional adaption to the nutritional change from a diet rich in fat (milk) to a diet rich in carbohydrates on from weaning. Time-resolved studies of intestinal development have so far been limited to investigation at the transcription level or to single or few proteins at a time. In the present study, we elucidate proteomic changes of primary intestinal epithelial cells from jejunum during early suckling (1-7 days of age), middle suckling (7-14 days), and weaning period (14-35 days) in mice, using a label-free proteomics approach. We show differential expression of 520 proteins during intestinal development and a pronounced change of the proteome during the middle suckling period and weaning. Proteins involved in several metabolic processes were found differentially expressed along the development. The temporal expression profiles of enzymes of the glycolysis were found to correlate with the increase in carbohydrate uptake at weaning, whereas the abundance changes of proteins involved in fatty acid metabolism as well as lactose metabolism indicated a nondiet driven preparation for the nutritional change at weaning. Further, we report the developmental abundance changes of proteins playing a vital role in the neonatal acquisition of passive immunity. In addition, different isoforms of several proteins were quantified, which may contribute to a better understanding of the roles of the specific isoforms in the small intestine. In summary, we provide a first, time-resolved proteome profile of intestinal epithelial cells along postnatal intestinal development. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.005231, 1-12, 2011.