Prevalence and distribution of ret/ptc 1, 2, and 3 in papillary thyroid carcinoma in New Caledonia and Australia
Prevalence and distribution of ret/ptc 1, 2, and 3 in papillary thyroid carcinoma in New Caledonia and Australia
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DOI：
10.1210/jc.85.8.2733
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发表时间：
2000-08-01
影响因子：
5.8
通讯作者：
Dong, QH
中科院分区：
文献类型：
--
作者：
Chua, EL;Wu, WM;Dong, QH
据报道，世界上甲状腺癌发病率最高的是位于澳大利亚和斐济之间的太平洋上的法国海外领地新喀里多尼亚的女性。到目前为止，还没有关于这一群体的分子遗传学研究。在过去的几年里，ret原癌基因（ret/ptc）的致癌重排在不同人群的乳头状癌中进行了研究。在这项研究中，我们调查了ret/ptc1, 2和3在新喀里多尼亚人群乳头状甲状腺癌中的患病率和分布，并将其与澳大利亚人群的模式进行了比较。本研究采用RT-PCR方法检测了27例新喀里多尼亚和20例澳大利亚患者新鲜冷冻和石蜡包埋的乳头状癌的ret重排，引物位于嵌合区两侧，然后与放射性探针杂交。ret/ptc存在于70%的新喀里多尼亚和85%的澳大利亚样本中。19例经测序发现并确认多重重排，其中4例在同一肿瘤中有3种重排。这项研究表明，ret/ptc在新喀里多尼亚和澳大利亚乳头状癌中具有很高的患病率。在同一肿瘤中发现多个ret/ptc提示某些甲状腺肿瘤可能确实是多克隆的。
The world's highest incidence of thyroid cancer has been reported among females in New Caledonia, a French overseas territory in the Pacific located between Australia and Fiji. To date, no molecular genetic studies in this population are available. Over the past few years, the oncogenic rearrangement of the ret protooncogene (ret/ptc) has been studied in papillary carcinomas in different populations. In this study, we investigated the prevalence and distribution of ret/ptc1, 2, and 3 in papillary thyroid carcinoma from the New Caledonian population and compared the pattern with that of an Australian population. Fresh-frozen and paraffin-embedded papillary carcinomas from 27 New Caledonian and 20 Australian patients were examined for ret rearrangements by means of RT-PCR with primers flanking the chimeric region, followed by hybridization with radioactive probes. ret/ptc was present in 70% of the New Caledonian and in 85% of the Australian samples. Multiple rearrangements were detected and confirmed by sequencing in 19 cases, 4 of which had 3 types of rearrangements in the same tumor. This study demonstrates a high prevalence of ret/ptc in New Caledonian and Australian papillary carcinoma. The findings of multiple ret/ptc in the same tumor suggest that some thyroid neoplasms may indeed be polyclonal.