Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat.
Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat.
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DOI：
10.1371/journal.pone.0154609
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
David J
中科院分区：
文献类型：
--
作者：
Holtz Y;Ardisson M;Ranwez V;Besnard A;Leroy P;Poux G;Roumet P;Viader V;Santoni S;David J
靶向序列捕获是一种有前途的技术，其有助于降低对大量个体中的许多基因组区域进行测序和基因分型的成本。设计诱饵序列以捕获先前在亲本或参考群体中发现的特定等位基因。我们研究了一组135个RILs起源于一个二粒小麦品种（Dic 2）和最近的硬粒小麦优良品种（Silur）之间的杂交。设计了6000个序列诱饵，以靶向先前RNAseq研究中发现的Dic 2与Silur多态性。将这些诱饵暴露于RIL群体的基因组DNA。回收了80%的目标SNP，其中65%具有高质量和覆盖率。最终的高密度遗传图谱由3,000多个标记组成，其遗传和物理图谱与大阵列获得的图谱一致。
Targeted sequence capture is a promising technology which helps reduce costs for sequencing and genotyping numerous genomic regions in large sets of individuals. Bait sequences are designed to capture specific alleles previously discovered in parents or reference populations. We studied a set of 135 RILs originating from a cross between an emmer cultivar (Dic2) and a recent durum elite cultivar (Silur). Six thousand sequence baits were designed to target Dic2 vs. Silur polymorphisms discovered in a previous RNAseq study. These baits were exposed to genomic DNA of the RIL population. Eighty percent of the targeted SNPs were recovered, 65% of which were of high quality and coverage. The final high density genetic map consisted of more than 3,000 markers, whose genetic and physical mapping were consistent with those obtained with large arrays.