Aperiodic capillary electrophoresis method using an alternating current electric field for separation of macromolecules
Aperiodic capillary electrophoresis method using an alternating current electric field for separation of macromolecules
复制标题
利用交流电场的非周期毛细管电泳法分离大分子
DOI：
10.1002/elps.200410408
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发表时间：
2005
期刊：
影响因子：
2.9
通讯作者：
S. Dukhin
中科院分区：
文献类型：
--
作者：
A. Dukhin;S. Dukhin
从直流（DC）到交流（AC）电场的转换为使用电场操纵各种液体系统的各种仪器技术提供了实质性的改进。例如，交流电场现在用于光散射和电声仪器中测量ξ电位，在很大程度上取代了使用直流电场的更传统的微电泳技术。在本文中，我们提出了一种新的方法来实现分离技术领域的类似转变，特别是毛细管电泳（CE）。介质电泳是一种众所周知的分离效应，在“空间非均匀”的交流电场中产生粒子的漂移运动。然而，还有另一种场效应也会导致类似的粒子漂移。这种方法依靠的不是“空间非均匀”场，而是“时间非均匀”场，通常被称为“非周期电泳”。尽管最近发表的一些实验和理论论文描述了这种效应，但它不如介电电泳为人所知。我们对一些相关论文做一个简短的概述。我们首次提出了“非周期电泳”可能用于大分子分离的想法。我们提出了几种新的机制，可以在足够强的交流电场中诱导这种效应。这种效应可以作为一种新的分离方法的基础，它比传统的CE有几个重要的优点。我们给出了一个简单的方案作为例子来说明这种新方法。
Switching from direct current (DC) to alternating current (AC) electric fields has provided substantial improvements in various instrument techniques that use electric fields for manipulating with various liquid‐based systems. For example, AC fields are now used in both light scattering and electroacoustic instruments for measuring ξ‐potential, largely replacing more traditional microelectrophoresis techniques that use DC fields. In this paper, we suggest a novel way to make a similar transition in the area of separation techniques, capillary electrophoresis (CE) in particular. Dielectrophoresis is one well‐known separation effect in which a drifting motion of particles is produced in a “spatially nonhomogeneous” AC electric field. However, there is another field effect that also causes a similar drift of particles. Instead of a “spatially nonhomogeneous” field, this method relies on a “temporally nonhomogeneous” field, normally referred to as “aperiodic electrophoresis”. Despite a number of recently published experimental and theoretical papers describing this effect, it is less well‐known than dielectrophoresis. We present a short overview of some of the relevant papers. We point out for the first time the idea that “aperiodic electrophoresis” might be useful for separation of macromolecules. We suggest several new mechanisms that could induce this effect in a sufficiently strong AC electric field. This effect can be used as a basis for a new separation method having several important advantages over traditional CE. We present a simple scheme as an example illustrating this new method.