Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana
Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana
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DOI：
10.1046/j.1365-313x.2000.00672.x
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发表时间：
2000-02-01
期刊：
影响因子：
7.2
通讯作者：
Rüdiger, W
中科院分区：
文献类型：
--
作者：
Oster, U;Tanaka, R;Rüdiger, W
叶绿素（Chl）的生物合成和降解是地球上唯一可以从卫星或其他行星上直接观察到的生物化学过程。大部分叶绿素存在于光合生物的捕光天线复合体中。令人惊讶的是，对Chl B的生物合成知之甚少，Chl B是Chl a之后第二丰富的Chl色素。本文描述了拟南芥叶绿素a加氧酶基因（CAO）的表达和性质，该基因显然是叶绿素B生物合成的关键酶。大肠杆菌中产生的重组酶催化一种不寻常的两步加氧酶反应，这是高等植物叶绿素循环中的“缺失环节”。
Chlorophyll (Chl) biosynthesis and degradation are the only biochemical processes on Earth that can be directly observed from satellites or other planets. The bulk of the Chls is found in the light-harvesting antenna complexes of photosynthetic organisms. Surprisingly little is known about the biosynthesis of Chl b, which is the second most abundant Chl pigment after Chl a. We describe here the expression and properties of the chlorophyllide a oxygenase gene (CAO) from Arabidopsis thaliana, which is apparently the key enzyme in Chl b biosynthesis. The recombinant enzyme produced in Escherichia coli catalyses an unusual two-step oxygenase reaction that is the 'missing link' in the chlorophyll cycle of higher plants.