Application of an enzyme-linked Immunosorbent assay for the analysis of paraquat in human-exposure samples
Application of an enzyme-linked Immunosorbent assay for the analysis of paraquat in human-exposure samples
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DOI：
10.1007/s00244-003-0251-x
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发表时间：
2005-02-01
影响因子：
4
通讯作者：
Hammock, BD
中科院分区：
文献类型：
--
作者：
Koivunen, ME;Gee, SJ;Hammock, BD
百草枯是一种有毒的季铵化合物，在世界各地用作除草剂。需要简单、快速、廉价但敏感的方法来研究长期、低水平接触百草枯对人类健康的影响。在对哥斯达黎加农场工人进行的一项人体接触研究中，采用酶联免疫吸附测定法（ELISA）对尿液和空气过滤器样本中的百草枯进行了定量分析。样品预处理包括使用固相萃取树脂柱去除干扰物质。使用一式两份加标尿样检测方法的精密度和准确度。使用ELISA和液相色谱-质谱法获得的盲样结果之间的相关性显著（加标和现场样品的R-2分别为0.945和0.906）。定量限为2 ng/mL（-1）时，该ELISA方法能够区分暴露与非暴露的农场工人。对于空气过滤器分析，百草枯在60 ℃下用9 M H2SO 4萃取12小时，ELISA获得的结果显示与分光光度计（256 nm）测量结果具有良好的相关性（R-2 = 0.918）。酸稳定尿样中的百草枯非常稳定，在室温、4摄氏度或-20摄氏度下储存3个月期间，未检测到明显损失。
Paraquat is a toxic quaternary ammonium compound used as an herbicide around the world. Easy, fast, and inexpensive but sensitive methods are needed to study the effects of long-term, low-level exposure of paraquat on human health. An enzyme-linked immunosorbent assay (ELISA) was used for quantification of paraquat in urine and air-filter samples collected in a human-exposure study among farm workers in Costa Rica. A sample pretreatment consisted of removal of interfering substances using solid-phase extraction resin columns. The precision and accuracy of the method were tested using duplicate spiked urine samples. The correlation between results for blind samples obtained using ELISA and liquid chromatography-mass spectrometry was significant (R-2 = 0.945 and 0.906 for spiked and field samples, respectively). With an LOQ of 2 ng mL(-1), this ELISA method was able to distinguish the exposed from the nonexposed farm workers. For the air-filter analysis, paraquat was extracted by 9 M H2SO4 at 60 degrees C for 12 hours, and the results obtained by ELISA showed good correlation (R-2 = 0.918) with the spectrophotometric (256 nm) measurements. Paraquat in acid-stabilized urine samples was very stable, and no significant losses were detected during a 3-month storage at room temperature, at 4 degrees C, or at -20 degrees C.