Systematic identification of microRNA and messenger RNA profiles in hepatitis C virus-infected human hepatoma cells
Systematic identification of microRNA and messenger RNA profiles in hepatitis C virus-infected human hepatoma cells
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系统鉴定丙型肝炎病毒感染的人肝癌细胞中的 microRNA 和信使 RNA 谱
DOI：
10.1016/j.virol.2009.11.036
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发表时间：
2010-03-01
期刊：
影响因子：
3.7
通讯作者：
Yang, Wei
中科院分区：
文献类型：
--
作者：
Liu, Xiuying;Wang, Tianyi;Yang, Wei
为了研究HCV感染过程中宿主microRNAs（miRNAs）/mRNAs（mRNAs）表达的整体动态变化，本研究利用人肝癌细胞进行了全面的基因芯片分析。共鉴定了108种人类miRNAs和1247种mRNA，其表达水平响应于HCV感染而变化超过2.0倍。HCV感染后，miRNA的表达模式反映了miRNA调控宿主细胞生理和抗病毒机制的参与，而与代谢、细胞生长、细胞凋亡和细胞因子/趋化因子途径相关的差异调节基因占优势。此外，构建了差异表达的miRNAs与其预测靶点的反向调控关联。最后，差异表达的miRNAs如miR-24、miR-149*、miR-638和miR-1181被鉴定为参与HCV的进入、复制和繁殖。这些结果表明，结合miRNA和mRNA谱可能具有上级潜力，作为HCV感染的诊断和机制特征。(C)2009 Elsevier Inc. All rights reserved.
In order to investigate the global and dynamic host microRNAs (miRNAs)/ messenger RNAs (mRNAs) expression alteration during in vitro acute HCV infection, a comprehensive microarray analysis was performed using human hepatoma cells. Totally, 108 human miRNAs and 1247 mRNAs were identified whose expression levels changed for more than 2.0-fold in response to HCV infection. Upon HCV infection, signature from the unique miRNA expression pattern reflected the involvement of miRNA-regulated host cellular physiology and antiviral mechanism, whereas a preponderance of differentially regulated genes associated with metabolism, cell growth, apoptosis and cytokine/chemokine pathways. Furthermore, a reverse regulatory association of differentially expressed miRNAs and their predicted targets was constructed. Finally, the differentially expressed miRNAs such as miR-24, miR-149*, miR-638 and miR-1181 were identified to be involved in HCV entry, replication and propagation. These results suggest that combined miRNA and mRNA profiling may have superior potential as a diagnostic and mechanistic feature in HCV infection. (C) 2009 Elsevier Inc. All rights reserved.