Aptamer-facilitated biomarker discovery (AptaBiD)
Aptamer-facilitated biomarker discovery (AptaBiD)
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DOI：
10.1021/ja801951p
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发表时间：
2008-07-16
影响因子：
15
通讯作者：
Krylov, Sergey N.
中科院分区：
文献类型：
--
作者：
Berezovski, Maxim V.;Lechmann, Matthias;Krylov, Sergey N.
在这里，我们介绍了一种发现生物标记物的技术，其中(I)选择在处于不同状态的细胞表面差异表达的生物标记物的DNA适配子；(Ii)使用适配子从细胞中分离生物标记物；(Iii)通过质谱仪鉴定分离的生物标记物。这项技术被称为适体促进的生物标记物发现(AptaBiD)。AptaBiD被用来发现区分活的成熟和未成熟树突状细胞的表面生物标志物。我们在体外选择了两个DNA适配子池，它们与成熟和未成熟的树突状细胞特异性结合，强度相差约100倍。适配子池在流动和磁珠辅助的成熟细胞和未成熟细胞的分离中被证明是高效的。然后，两个适配子池被用来从细胞中分离生物标记物。随后对分离出的蛋白质进行了质谱分析，发现了未成熟和成熟树突状细胞的未知生物标记物。
Here we introduce a technology for biomarker discovery in which (i) DNA aptamers to biomarkers differentially expressed on the surfaces of cells being in different states are selected; (ii) aptamers are used to isolate biomarkers from the cells; and (iii) the isolated biomarkers are identified by means of mass spectrometry. The technology is termed aptamer-facilitated biomarker discovery (AptaBiD). AptaBiD was used to discover surface biomarkers that distinguish live mature and immature dendritic cells. We selected in vitro two DNA aptamer pools that specifically bind to mature and immature dendritic cells with a difference in strength of approximately 100 times. The aptamer pools were proven to be highly efficient in flow- and magnetic-bead-assisted separation of mature cells from immature cells. The two aptamer pools were then used to isolate biomarkers from the cells. The subsequent mass spectrometry analysis of the isolated proteins revealed unknown biomarkers of immature and mature dendritic cells.