Isolation of eight novel Caenorhabditis elegans small RNAs
Isolation of eight novel Caenorhabditis elegans small RNAs
复制标题
DOI：
10.1016/j.gene.2004.03.004
复制
发表时间：
2004-06-23
期刊：
影响因子：
3.5
通讯作者：
Ushida, C
中科院分区：
文献类型：
--
作者：
Wachi, M;Ogawa, T;Ushida, C
从秀丽隐杆线虫中分离到8个新的小RNA，它们编码蛋白质编码基因的内含子区域。其中7个具有典型的snoRNA二级结构：1个C/D snoRNA和6个H/ACA snoRNA。剩余的一种RNA与数据库中的其他RNA没有显示出任何同源性。7个分离的snoRNA中有4个能与部分rRNA形成碱基配对，提示它们是潜在的假尿嘧啶位点和甲基化位点。我们的研究结果表明，在C.优雅(C)2004 Elsevier B. V.保留所有权利。
Eight novel small RNAs that were encoded in the regions corresponding to the introns of protein-coding genes were isolated from Caenorhabditis elegans. Seven of them showed a typical snoRNA secondary structure: one C/D snoRNA and six H/ACA snoRNAs. The remaining one RNA did not show any homology to other RNAs in a database. Four of the seven isolated snoRNAs could form base pairings with parts of rRNAs, suggesting that they are potential pseudouridilation sites and methylation sites. The results of our study suggest that there are more as-yet-unidentified small ncRNAs of which genes are located in the intron regions of protein-coding genes in C. elegans. (C) 2004 Elsevier B.V. All rights reserved.