Antigout Effects of Plantago asiatica: Xanthine Oxidase Inhibitory Activities Assessed by Electrochemical Biosensing Method.
Antigout Effects of Plantago asiatica: Xanthine Oxidase Inhibitory Activities Assessed by Electrochemical Biosensing Method.
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车前子的抗痛风作用：通过电化学生物传感方法评估黄嘌呤氧化酶抑制活性
DOI：
10.1155/2018/1364617
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yuan CS
中科院分区：
文献类型：
--
作者：
Zeng JX;Wang J;Zhang SW;Zhu JX;Li M;Huang WH;Wan JY;Yao HQ;Wang CZ;Yuan CS
The XOD inhibitory effects of Plantaginis Semen, that is, the seeds of P. asiatisca, and its representative four single compounds, acteoside, 1H-indolo-3-carbaldehyde, isoacteoside, and myristic acid, were evaluated by electron transfer signal blocking activities (ETSBA), which is based on the electron transfer signal of XOD enzymatic reaction. The blocking activities were detected using an electrochemical biosensing method. Compared with control, significant effects were observed after the addition of P. asiatica extract, acteoside, and 1H-indolo-3-carbaldehyde (all p < 0.05). The IC50 values of the extract and acteoside are 89.14 and 7.55 μg·mL−1, respectively. The IC20 values of the extract, acteoside, and 1H-indolo-3-carbaldehyde are 24.28, 3.88, and 16.16 μg·mL−1, respectively. Due to the relatively lower inhibitory potential of 1H-indolo-3-carbaldehyde, its IC50 was not obtained. In addition, isoacteoside and myristic acid did not show any XOD inhibitory effects. Our data demonstrated that the XOD inhibitory effects of the extract, acteoside, and 1H-indolo-3-carbaldehyde can be accurately evaluated by the ETSBA method. The results from this study indicated that Plantaginis Semen significantly inhibited XOD activities to reduce hyperuricemia and treat gout. The study also proves that measuring the electron transfer signal blocking activities is a simple, sensitive, and accurate method to evaluate the XOD inhibitory effects.
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影响因子：
15
作者：
Aguey-Zinsou, KF;Bernhardt, PV;Leimkühler, S
通讯作者：
Leimkühler, S
DOI：
10.1006/bbrc.1996.1148
发表时间：
1996-08-05
影响因子：
3.1
作者：
Motterlini, R;Hidalgo, A;Green, CJ
通讯作者：
Green, CJ
影响因子：
1.6
作者：
Zeng, JX;Wei, WZ;Wu, L
通讯作者：
Wu, L
固定在玻碳电极上的黄嘌呤氧化酶/合成锂皂石纳米粒子：直接电子转移和多电催化
影响因子：
12.6
作者：
Shan, Dan;Wang, Yan-Na;Ding, Shou-Nian
通讯作者：
Ding, Shou-Nian
超滤液相色谱-逆流色谱联用筛选分离西洋参潜在黄嘌呤氧化酶抑制剂的方法
影响因子：
6.1
作者：
Li, Sainan;Tang, Ying;Zhang, Yuchi
通讯作者：
Zhang, Yuchi