In vivo calcium imaging of OFF-responding ASK chemosensory neurons in C. elegans
In vivo calcium imaging of OFF-responding ASK chemosensory neurons in C. elegans
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DOI：
10.1016/j.bbagen.2009.03.032
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发表时间：
2009-08-01
影响因子：
3
通讯作者：
Shingai, Ryuzo
中科院分区：
文献类型：
--
作者：
Wakabayashi, Tokumitsu;Kimura, Yukihiro;Shingai, Ryuzo
背景：神经元和神经元回路如何将感觉输入转化为行为尚不清楚。秀丽线虫因其简单的神经系统而成为研究这一问题的理想模式生物。将感觉信号转换为神经活动是动物神经系统感觉-运动转换途径中至关重要的第一步。方法：在线虫ASK神经元中表达一种基因编码的钙感受器蛋白G-cAMP，并观察其细胞内钙动态变化。结果：应用L赖氨酸或食物相关刺激剂后，线虫ASK神经元内钙离子水平降低。相反，在去除刺激后，反应会增加。结论：观察到的对外界刺激反应的变化提示ASK神经元的活动可能影响刺激诱发的蠕虫行为，ASK神经元的刺激开启/激活关闭特性与脊椎动物视网膜光感受器相似。一般意义：基于神经元回路的活动和结构分析感觉-运动转换通路是神经生物学的重要目标，对线虫具有实用价值。我们的研究为动物体内这种转化的机制提供了洞察力。(C)2009爱思唯尔B.V.保留所有权利。
Background: How neurons and neuronal circuits transform sensory input into behavior is not well understood. Because of its well-described, simple nervous system, Caenorhabditis elegans is an ideal model organism to study this issue. Transformation of sensory signals into neural activity is a crucial first step in the sensory-motor transformation pathway in an animal's nervous system. We examined the properties of chemosensory ASK neurons of C. elegans during sensory stimulation.Method: A genetically encoded calcium sensor protein, G-CaMP, was expressed in ASK neurons of C. elegans, and the intracellular calcium dynamics of the neurons were observed.Results: After application of the attractants L-lysine or food-related stimuli, the level of calcium in ASK neurons decreased. In contrast, responses increased upon stimulus removal. Opposite responses were observed after application and removal of a repellent.Conclusion: The observed changes in response to external stimuli suggest that the activity of ASK neurons may impact stimulus-evoked worm behavior The stimulus-ON/activity-OFF properties of ASK neurons are similar to those of vertebrate retinal photoreceptors.General significance: Analysis of sensory-motor transformation pathways based on the activity and structure of neuronal circuits is an important goal in neurobiology and is practical in C. elegans. Our study provides insights into the mechanism of such transformation in the animal. (C) 2009 Elsevier B.V. All rights reserved.