Organophosphate Resistance and Its Main Mechanism in Populations of Codling Moth (Lepidoptera: Tortricidae) from Central Chile
Organophosphate Resistance and Its Main Mechanism in Populations of Codling Moth (Lepidoptera: Tortricidae) from Central Chile
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DOI：
10.1093/jee/tou001
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
M. Reyes;W. Barros-Parada;C. Ramírez;E. Fuentes‐Contreras
中科院分区：
文献类型：
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作者：
M. Reyes;W. Barros-Parada;C. Ramírez;E. Fuentes‐Contreras
摘要 苹果蠹蛾（Cydia pomonella (L.)）是全球苹果生产的主要害虫。根据五种不同的机制，所有生产国都报告了杀虫剂抗药性。智利的苹果蠹蛾滞育后幼虫对谷硫磷和虫酰肼具有抗药性。然而，还没有关于这些人群对其他化学组杀虫剂敏感性的研究。因此，研究了谷硫磷、乙基毒死蜱、高氰戊菊酯、甲氧虫酰肼、虫酰肼和噻虫啉对六个田间种群的新生和滞育后幼虫的功效，并评估了该物种的抗性机制。新生幼虫对所研究的所有杀虫剂均敏感，但四个种群的滞育后幼虫对毒死蜱具有抗性，其中一种还对谷硫磷具有抗性，另一种对虫酰肼具有抗性。未检测到乙酰胆碱酯酶不敏感突变，并且钠通道敲低抗性突变在一个群体中以较低频率存在。成人中谷胱甘肽 S-转移酶、酯酶和细胞色素 P-450 单加氧酶的解毒酶活性因人群而异，但毒死蜱耐药性仅与酯酶活性降低相关，如毒死蜱死亡率与酯酶活性之间显着负相关所示。
ABSTRACT The codling moth, Cydia pomonella (L.), is the key pest of apple production worldwide. Insecticide resistance has been reported in all producing countries, based on five different mechanisms. Codling moth in Chile has resistance to azinphos-methyl and tebufenozide in post-diapausing larvae. However, there are no studies about the susceptibility of these populations to insecticides from other chemical groups. Therefore, the efficacy of azinphos-methyl, chlorpyrifos-ethyl, esfenvalerate, methoxyfenozide, tebufenozide, and thiacloprid on neonate and post-diapausing larvae from six field populations was investigated, and identified resistance mechanisms in this species were evaluated. Neonate larvae were susceptible to all insecticides studied, but post-diapausing larvae from four populations were resistant to chlorpyrifos, one of them was also resistant to azinphos-methyl, and another one was resistant to tebufenozide. The acetylcholinesterase insensitivity mutation was not detected, and the sodium channel knockdown resistance mutation was present in a low frequency in one population. Detoxifying enzymatic activity of glutathione S-transferases, esterases, and cytochrome P-450 monooxygenases in adults differed among populations, but chlorpyrifos resistance was associated only with a decreased esterase activity as shown by a significant negative correlation between chlorpyrifos mortality and esterase activity.