Dorsomedial hypothalamic corticotropin-releasing factor mediation of exercise-induced anorexia
Dorsomedial hypothalamic corticotropin-releasing factor mediation of exercise-induced anorexia
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DOI：
10.1152/ajpregu.00805.2004
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发表时间：
2005-06-01
影响因子：
2.8
通讯作者：
Bi, S
中科院分区：
文献类型：
--
作者：
Kawaguchi, M;Scott, KA;Bi, S
跑轮和随之而来的自愿锻炼改变了食物的摄入量，减轻了体重。这些效应背后的神经机制尚不清楚。在这项研究中，我们首先评估了7天的跑轮接触对食物摄入量、体重和下丘脑基因表达的影响。我们证明，接近转轮显着减少摄食量和体重，并导致下丘脑背内侧(DMH)CRF mRNA表达显着增加，但室旁核不表达。七天的跑轮接触也会导致弓状核和DMH神经肽Y基因表达的升高。为了评估DMH CRF活性升高在运动诱导的食物摄入量和体重变化中的潜在作用，我们比较了接受脑室(ICV)CRF拮抗剂α-螺旋CRF或带有或不带有转轮的车辆的大鼠摄食量、体重和下丘脑基因表达的变化。在为期4天的转轮运动期间，我们发现，侧脑室注射CRF拮抗剂显著减轻了运动引起的食物摄入量和体重的减少。对食物摄入量的影响是由于阻断了运动引起的膳食大小变化而产生的。中枢CRF拮抗剂注射进一步增加运动大鼠DMH CRF基因的表达。综上所述，这些数据表明DMH CRF在自愿运动增加引起的厌食症中起着关键作用。
Running wheel access and resulting voluntary exercise alter food intake and reduce body weight. The neural mechanisms underlying these effects are unclear. In this study, we first assessed the effects of 7 days of running wheel access on food intake, body weight, and hypothalamic gene expression. We demonstrate that running wheel access significantly decreases food intake and body weight and results in a significant elevation of CRF mRNA expression in the dorsomedial hypothalamus (DMH) but not the paraventricular nucleus. Seven-day running wheel access also results in elevated arcuate nucleus and DMH neuropeptide Y gene expression. To assess a potential role for elevated DMH CRF activity in the activity-induced changes in food intake and body weight, we compared changes in food intake, body weight, and hypothalamic gene expression in rats receiving intracerebroventricular (ICV) CRF antagonist alpha-helical CRF or vehicle with or without access to running wheels. During a 4-day period of running wheel access, we found that exercise-induced reductions of food intake and body weight were significantly attenuated by ICV injection of the CRF antagonist. The effect on food intake was specific to a blockade of activity-induced changes in meal size. Central CRF antagonist injection further increased DMH CRF mRNA expression in exercised rats. Together, these data suggest that DMH CRF play a critical role in the anorexia resulting from increased voluntary exercise.