Chaotropic solutes cause water stress in Pseudomonas putida
Chaotropic solutes cause water stress in Pseudomonas putida
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DOI：
10.1111/j.1462-2920.2003.00478.x
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发表时间：
2003-12-01
影响因子：
5.1
通讯作者：
Timmis, KN
中科院分区：
文献类型：
--
作者：
Hallsworth, JE;Heim, S;Timmis, KN
水资源利用率低是陆地植物、动物和微生物面临压力的最普遍原因，并对生态系统功能和农业生产力产生重大影响。对水分胁迫的研究主要集中在影响细胞膨胀的条件上，即诱导渗透胁迫。我们发现，在一种典型的土壤细菌——腐臭假单胞菌中，不影响膨胀的混沌性溶质会降低水活性，扰乱大分子与水的相互作用，从而破坏细胞大分子的稳定，抑制生长，是水胁迫的有力介质。朝向溶质诱导的水分胁迫主要导致与生物大分子和膜结构稳定有关的蛋白质的上调。许多环境污染物和农产品都是混沌性化学物质，因此构成了水体和土壤中一种以前未被认识到但常见的生物应激形式。
Low water availability is the most ubiquitous cause of stress for terrestrial plants, animals and microorganisms, and has a major impact on ecosystem function and agricultural productivity. Studies of water stress have largely focused on conditions that affect cell turgor, i.e. induce osmotic stress. We show that chaotropic solutes that do not affect turgor reduce water activity, perturb macromolecule-water interactions and thereby destabilize cellular macromolecules, inhibit growth, and are powerful mediators of water stress in a typical soil bacterium, Pseudomonas putida. Chaotropic solute-induced water stress resulted mostly in the upregulation of proteins involved in stabilization of biological macromolecules and membrane structure. Many environmental pollutants and agricultural products are chaotropic chemicals and thus constitute a previously unrecognised but common form of biological stress in water bodies and soils.