SOD3 acts as a tumor suppressor in PC-3 prostate cancer cells via hydrogen peroxide accumulation.
SOD3 acts as a tumor suppressor in PC-3 prostate cancer cells via hydrogen peroxide accumulation.
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发表时间：
2014-06
影响因子：
2
通讯作者：
Jungim Kim;A. Mizokami;Minkyoung Shin;K. Izumi;H. Konaka;Y. Kadono;Y. Kitagawa;E. Keller;Jian Zhang;M. Namiki
中科院分区：
文献类型：
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作者：
Jungim Kim;A. Mizokami;Minkyoung Shin;K. Izumi;H. Konaka;Y. Kadono;Y. Kitagawa;E. Keller;Jian Zhang;M. Namiki
超氧化物歧化酶-3（SOD-3）作用于细胞表面，保护细胞免受氧化应激，其在前列腺癌进展中的作用尚不清楚。材料和方法为了验证SOD 3在人类前列腺组织中的表达，使用组织微阵列进行免疫组织化学。为了研究SOD3对增殖、迁移和侵袭的影响，将SOD3过表达并将重组SOD3用于PC-3前列腺癌细胞中。在SOD3过表达的PC-3细胞中估计H2O2水平、还原型谷胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）比率、过氧化氢酶活性和8-氧代-2'-脱氧鸟苷（8-OHdG）。结果免疫组化显示前列腺癌组织中SOD3表达减少。PC-3细胞中SOD 3过表达抑制细胞增殖、迁移和侵袭。重组SOD 3具有相同的效果。SOD 3过表达增加H2 O2积累，GSH/GSSG比值降低，过氧化氢酶活性降低。8-OHdG升高证实了SOD 3过表达细胞中的DNA损伤。结论由于SOD3具有抑癌作用，SOD3过表达及重组SOD3可能为前列腺癌的治疗提供新的思路。
BACKGROUND The functions of superoxide dismutase-3 (SOD3), which acts on the cell surface and protects cells from oxidative stress, remain uncertain in the progression of prostate cancer. MATERIALS AND METHODS To verify SOD3 expression in human prostate tissue, immunohistochemistry was performed using tissue microarrays. To investigate the effects of SOD3 on proliferation, migration, and invasion, SOD3 was overexpressed and recombinant SOD3 was employed in PC-3 prostate cancer cells. H2O2 levels, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, catalase activity, and 8-oxo-2'-deoxyguanosine (8-OHdG) were estimated in SOD3-overexpressing PC-3 cells. RESULTS Immunohistochemistry revealed reduced expression of SOD3 in prostate cancer tissue. SOD3 overexpression in PC-3 cells inhibited cell proliferation, migration, and invasion. Recombinant SOD3 had the same effect. H2O2 accumulation was increased by SOD3 overexpression, GSH/GSSG ratio was decreased, and catalase activity was decreased. DNA damage in SOD3-overexpressing cells was confirmed by 8-OHdG elevation. CONCLUSION Since SOD3 acts as a tumor suppressor, SOD3 overexpression and recombinant SOD3 might lead to treatment for prostate cancer.