The effect of Mycoplasma gallisepticum infection on energy metabolism in chicken lungs: Through oxidative stress and inflammation
The effect of Mycoplasma gallisepticum infection on energy metabolism in chicken lungs: Through oxidative stress and inflammation
复制标题
鸡毒支原体感染对鸡肺能量代谢的影响：通过氧化应激和炎症
DOI：
10.1016/j.micpath.2019.103848
复制
发表时间：
2020-01-01
影响因子：
3.8
通讯作者：
Li, Jichang
中科院分区：
文献类型：
--
作者：
Ishfaq, Muhammad;Zhang, Wei;Li, Jichang
鸡毒支原体 (Mg) 会导致鸡慢性呼吸道疾病 (CRD)。然而，镁感染对鸡肺能量代谢的影响尚不清楚。本研究旨在探讨镁感染对鸡肺能量代谢的影响。将4周龄白来亨鸡随机分为对照组(L1)和镁感染组(L2)。使用组织病理学、透射电子显微镜、qRT-PCR 和蛋白质印迹来确定超微结构分析、炎症和能量代谢的特征。结果显示，镁感染引起鸡肺氧化应激，血清细胞因子活性在三个时间点均增强。感染镁的鸡表现出异常的形态和细胞损伤，包括肺部炎症细胞浸润增加、细胞碎片和渗出物、线粒体和 DNA 损伤。 L2组炎症相关基因的mRNA和蛋白表达水平显着升高，表明Mg诱导了鸡肺炎症。此外，与L1组相比，L2组的ATP酶活性降低。同时，在所有评估时间点，能量代谢相关基因的mRNA和蛋白水平表达均下降，这表明镁感染削弱了鸡肺的能量代谢。综上所述，数据提示镁感染引起鸡肺氧化应激、炎症和能量代谢功能障碍，探索新的治疗靶点，为比较兽医学提供参考。
Mycoplasma gallisepticum (Mg) causes chronic respiratory disease (CRD) in chickens. However, the effect of Mg infection on energy metabolism in chicken lungs is still unknown. The present study was aimed to investigate the effect of Mg infection on energy metabolism in chicken lungs. Four-weeks-old white leghorn chickens were randomly divided into control group (L1) and Mg infection group (L2). Histopathology, transmission electron microscopy, qRT-PCR and Western blot were used to determine the hallmarks of ultrastructural analysis, inflammation and energy metabolism. Results revealed that Mg infection induced oxidative stress in the chicken lungs and serum cytokine activities were enhanced at the three time points. Chickens infected with Mg revealed abnormal morphology and cellular damage including increased inflammatory cells infiltrate, cellular debris and exudate, mitochondrial and DNA damage in the lungs. The mRNA and protein expression level of inflammation-related genes were significantly increased in L2 group, showing that Mg induced inflammation in chicken lungs. In addition, ATPase activities were reduced in L2 group compared to L1 group. Meanwhile, the expression of energy metabolism related genes were decreased at both mRNA and protein level at all assessed time points, which showed that Mg infection weakened energy metabolism in chicken lungs. In summary, the data suggested that Mg infection induced oxidative stress, inflammation and energy metabolism dysfunction in the chicken lungs, exploring new therapeutic targets and providing a reference for comparative veterinary medicine.