A receptor heteromer mediates the male perception of female attractants in plants
A receptor heteromer mediates the male perception of female attractants in plants
复制标题
受体异聚体介导植物中雄性对雌性引诱剂的感知
DOI：
10.1038/nature16975
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发表时间：
2016-03-10
期刊：
影响因子：
64.8
通讯作者：
Yang, Wei-Cai
中科院分区：
文献类型：
--
作者：
Wang, Tong;Liang, Liang;Yang, Wei-Cai
有性生殖需要雌雄配子之间的识别。在有花植物中，不动的精子通过引导花粉管生长被运送到胚珠包围的雌配子体中。虽然雌配子体分泌的肽类物质已被鉴定为对花粉管具有趋化作用，但雄配子体的受体尚不清楚。在这里，我们确定了一个细胞表面受体异聚体，MDIS 1-MIK，在花粉管上感知雌性引诱剂LURE 1在拟南芥。MDIS 1、MIK 1和MIK 2是定位于质膜的受体样激酶，具有细胞外富含亮氨酸的重复序列和细胞内激酶结构域。LURE 1特异性结合MDIS 1、MIK 1和MIK 2的胞外结构域，而MDIS 1和MIK 1、MIK 2突变体的花粉管对LURE 1的反应较不敏感。此外，LURE 1触发受体的二聚化并激活MIK 1的激酶活性。重要的是，AtMDIS 1转化到姐妹种红苋菜中部分打破了生殖隔离屏障。我们的研究结果揭示了雄性感知雌性吸引信号的一种新机制。
Sexual reproduction requires recognition between the male and female gametes. In flowering plants, the immobile sperms are delivered to the ovule-enclosed female gametophyte by guided pollen tube growth. Although the female gametophyte-secreted peptides have been identified to be the chemotactic attractant to the pollen tube,,, the male receptor(s) is still unknown. Here we identify a cell-surface receptor heteromer, MDIS1–MIK, on the pollen tube that perceives female attractant LURE1 inArabidopsis thaliana. MDIS1, MIK1 and MIK2 are plasma-membrane-localized receptor-like kinases with extracellular leucine-rich repeats and an intracellular kinase domain. LURE1 specifically binds the extracellular domains of MDIS1, MIK1 and MIK2, whereasmdis1andmik1 mik2mutant pollen tubes respond less sensitively to LURE1. Furthermore, LURE1 triggers dimerization of the receptors and activates the kinase activity of MIK1. Importantly, transformation of AtMDIS1to the sister speciesCapsella rubellacan partially break down the reproductive isolation barrier. Our findings reveal a new mechanism of the male perception of the female attracting signals.