CHROMATOGRAPHIC-SEPARATION OF VENOM OF EGYPTIAN BLACK SNAKE (WALTERINNESIA-AEGYPTIA) AND PHARMACOLOGICAL CHARACTERIZATION OF ITS COMPONENTS
CHROMATOGRAPHIC-SEPARATION OF VENOM OF EGYPTIAN BLACK SNAKE (WALTERINNESIA-AEGYPTIA) AND PHARMACOLOGICAL CHARACTERIZATION OF ITS COMPONENTS
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DOI：
10.1016/0041-0101(76)90023-4
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发表时间：
1976-01-01
期刊：
影响因子：
2.8
通讯作者：
MEBS, D
中科院分区：
文献类型：
--
作者：
LEE, CY;CHEN, YM;MEBS, D
从中华白蛇毒中分离得到三种神经毒性成分（W-III、W-IV和W-V）。通过CM-纤维素柱层析，然后在Sephadex G-50柱上凝胶过滤，分离埃及伊蚊。小鼠（i. p.）对于W-III为0.95 μ g/g体重，对于W-IV为0.14 μ g/g，对于W-V为0.2 μ g/g，而在雏鸡中（i.m.）W-III超过0.5 μ g/g，W-IV超过0.12 μ g/g，W-V超过0.23 μ g/g。所有3种毒素都阻断离体大鼠膈神经-膈肌和鸡颈二腹肌的神经肌肉传递，并抑制后者对乙酰胆碱的反应，就像其他突触后作用的蛇神经毒素一样。由W-III产生的神经肌肉阻滞在两种肌肉制备物中是可逆的，而由W-IV以及W-V产生的神经肌肉阻滞在鸡双腹颈中是不可逆的，但在大鼠膈肌中部分可逆。
Three neurotoxic components (W-III, W-IV and W-V) were isolated from the venom of W. aegyptia by CM-cellulose column chromatography, followed by gel filtration on a Sephadex G-50 column. The LD50 in mice (i.p.) was 0.95 .mu.g/g body weight for W-III, 0.14 .mu.g/g for W-IV and 0.2 .mu.g/g for W-V, while in baby chicks (i.m.) it was over 0.5 .mu.g/g for W-III, 0.12 .mu.g/g for W-IV and 0.23 .mu.g/g for W-V. All of the 3 toxins blocked the neuromuscular transmission of the isolated rat phrenic nerve-diaphragm and the chick biventer cervicis muscle and inhibited the response of the latter to acetylcholine, just like other postsynaptically acting snake neurotoxins. The neuromuscular block produced by W-III was reversible in both muscle preparations, whereas that by W-IV as well as W-V was irreversible in the chick biventer cervics but partially reversible in the rat diaphragm.