Diffusion tensor magnetic resonance imaging of rat glioma models: a correlation study of MR imaging and histology.
Diffusion tensor magnetic resonance imaging of rat glioma models: a correlation study of MR imaging and histology.
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DOI：
10.1097/rct.0b013e3182685436
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发表时间：
2012-11
影响因子：
1.3
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Wang S;Zhou J
扩散张量磁共振成像(DTI)可以用来表征有序生物组织的微观结构。本研究旨在利用弥散张量成像评价大鼠脑胶质瘤及其周围脑组织的组织学特征。3种类型的肿瘤，9L胶质肉瘤(n=8)，F98胶质瘤(n=5)和人脑胶质母细胞瘤异种移植瘤(GBM22；n=8)在大鼠脑内孵育，用4.7T动物MRI系统进行常规MRI和DTI扫描。分析各向异性分数(FA)、各向同性表观扩散系数(ADc)、平行扩散系数(λ//)和垂直扩散系数(λ┴)，以及几个感兴趣区的组织学特征。所有肿块均由低FA中心区(肿瘤中心)和高FA周边区(肿瘤边缘)组成。组织学检查显示肿瘤边缘有高度粘连的肿瘤组织(9L和F98为圆形，GBM22为放射状)。瘤周水肿区的ADC值、λ┴和λ//均高于对侧灰质。9L和F98肿瘤同侧脑白质FA明显低于对侧，λ┴明显高于对侧，而9L和F98无明显差异。组织学检查显示，GBM22肿瘤侵袭同侧受损白质。DTI指标的定量分析为评估肿瘤的微结构和肿瘤细胞对邻近灰质和白质的侵袭提供了有用的信息。
Diffusion tensor MR imaging (DTI) can be used to characterize the microstructures of ordered biological tissues. This study was designed to assess histological features of gliomas and surrounding brain tissues in rats using DTI. Three types of tumors, a 9L gliosarcoma (n = 8), an F98 glioma (n = 5), and a human glioblastoma xenograft (GBM22; n = 8) were incubated in rat brains and underwent conventional MRI and DTI scanning using a 4.7 T animal MRI system. Fractional anisotropy (FA), isotropic apparent diffusion coefficient (ADC), parallel diffusivity (λ//), and perpendicular diffusivity (λ┴), as well as histological features within several regions of interest, were analyzed. All tumor masses consisted of low-FA central zones (tumor center) and high-FA peripheral regions (tumor rim). Histological examination revealed the existence of highly coherent tumor organizations (circular for 9L and F98 or radial for GBM22) in the tumor rims. There were higher ADC, λ┴ and λ// in the peritumoral edema, compared to the contralateral grey matter. There were significantly lower FA and higher λ┴ in the ipsilateral white matter than in the contralateral white matter for the GBM22 tumor, whereas there were no differences for the 9L and F98 tumors. Histology showed GBM22 tumor infiltration into the ipsilateral damaged white matter. Quantitative analysis of DTI indices provides useful information for assessing tumor microstructures and tumor cell invasion into the adjacent gray matter and white matter.