Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells
Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells
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DOI：
10.1016/j.bbrc.2008.07.111
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发表时间：
2008-10-10
影响因子：
3.1
通讯作者：
Suemori, Hirofumi
中科院分区：
文献类型：
--
作者：
Miyazaki, Takamichi;Futaki, Sugiko;Suemori, Hirofumi
人类胚胎干细胞（hESCs）被认为是细胞移植治疗的一种有前途的细胞来源。对于这样的临床应用，hESC应使用适当且合格的材料进行操作。在这项研究中，我们研究了重组人层粘连蛋白（rhLM）异构体油的未分化的hESC的生长的功效。我们首先确定了hESC上表达的主要整合素以揭示hESC对rhLM的偏好，并发现hESC主要表达整合素α 6 β 1，其主要结合层粘连蛋白-111、-332和-511/-521。当将hESC接种到rhLM上时，细胞确实显著粘附于rhLM-332，并且粘附于rhLM-511和rhLM-111的程度较低。hESC在这三种rhLM上增殖了几代，同时保留了它们的多能性。这些结果表明rhLM-111. -332和-511是扩增未分化hESC的良好底物，因为它们对hESC上表达的整合素α 6 β 1具有高亲和力。版权所有（C）2008 Elsevier Inc. All rights reserved.
Human embryonic stem cells (hESCs) are thought to be a promising cell source for cell transplantation therapy. For such a clinical application, the hESCs should be manipulated using appropriate and qualified materials. In this study, we examined the efficacy of recombinant human laminin (rhLM) isoforms oil the undifferentiated growth of hESCs. We first determined the major integrins expressed on the hESCs to reveal the preference of the hESCs for rhLMs, and found that the hESCs mainly expressed integrin alpha 6 beta 1, which binds predominantly to laminin-111, -332 and -511/-521. When the hESCs were seeded onto rhLMs, the cells indeed adhered markedly to rhLM-332, and to rhLM-511 and rhLM-111 to a lesser extent. The hESCs proliferated on these three rhLMs for several passages while preserving their pluripotency. These results show that rhLM-111. -332, and -511 are good substrates to expand undifferentiated hESCs due to their high affinity to integrin alpha 6 beta 1 expressed on hESCs. Crown copyright (C) 2008 Published by Elsevier Inc. All rights reserved.