Subcloning, crystallization and preliminary X-ray analysis of the signal receiver domain of ETR1, an ethylene receptor from Arabidopsis thaliana.
Subcloning, crystallization and preliminary X-ray analysis of the signal receiver domain of ETR1, an ethylene receptor from Arabidopsis thaliana.
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ETR1（拟南芥乙烯受体）信号接收域的亚克隆、结晶和初步 X 射线分析。
DOI：
10.1107/s0907444997015023
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
S. Kim
中科院分区：
文献类型：
--
作者：
A. Grantz;H. J. Müller;S. Kim
拟南芥（Arabidopsis thaliana）乙烯受体ETR1的信号受体结构域在大肠杆菌中亚克隆表达，并通过亲和层析纯化。已经获得了接收域的原生和硒代蛋氨酸取代形式的晶体。原生晶体生长在1.6 M Li2SO4和0.1 M HEPES pH 7中。5、一次闪冻衍射至2.1 A分辨率。它们属于空间群P41212，单元格尺寸a = b = 48.4, c = 112.3 a。
The signal receiver domain of ETR1, an ethylene receptor from Arabidopsis thaliana, has been subcloned and expressed in E. coli and purified by affinity chromatography. Crystals of both native and a selenomethionine-substituted form of the receiver domain have been obtained. Native crystals grew in 1.6 M Li2SO4 and 0.1 M HEPES pH 7. 5 and once flash-frozen diffract to 2.1 A resolution. They belong to space group P41212 with unit-cell dimensions a = b = 48.4, c = 112.3 A.