A slow tritium exchange study of the solution structure of Escherichia coli 5 S ribosomal RNA.
A slow tritium exchange study of the solution structure of Escherichia coli 5 S ribosomal RNA.
复制标题
大肠杆菌 5S 核糖体 RNA 溶液结构的缓慢氚交换研究。
DOI：
10.1016/0022-2836(81)90433-2
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发表时间：
1981
影响因子：
5.6
通讯作者：
Cantor,CR
中科院分区：
文献类型：
--
作者：
Farber,NM;Cantor,CR
通过缓慢的氚交换研究了大肠杆菌5S核糖体RNA的结构。 5 S rRNA 在 37 °C 下在含有 3H2O 的缓冲液中孵育，通过酶消化和测量分离片段的比活性来确定氚从溶剂中掺入特定位点的情况。标记率对微环境非常敏感，并且可以绘制分子的构象图。整个分子中分布的位点的交换率允许对所有提出的 5 S rRNA 模型进行严格评估。虽然数据支持 Fox & Woese (1975) 模型中的四个基本螺旋，但还指出了其他相互作用区域。描述了一种改进的氚交换方法的双标记方法。
The structure ofEscherichia coli5 S ribosomal RNA has been investigated by slow tritium exchange. 5 S rRNA was incubated in a3H2O-containing buffer at 37 °C and the incorporation of tritium from the solvent into specific sites was determined by enzymatic digestion and measurement of the specific activity of the isolated fragments. The labeling rate is markedly sensitive to the microenvironment and permits mapping the conformation of the molecule. The exchange rate into sites distributed throughout the molecule permits a critical evaluation of all proposed 5 S rRNA models. While the data support the four basic helices in the Fox & Woese (1975) model, additional regions of interaction are indicated. An improved dual-label approach to the tritium exchange method is described.