BIOSYNTHESIS OF EGG-LAYING HORMONE (ELH) IN BAG CELL NEURONS OF APLYSIA-CALIFORNICA
BIOSYNTHESIS OF EGG-LAYING HORMONE (ELH) IN BAG CELL NEURONS OF APLYSIA-CALIFORNICA
复制标题
DOI：
10.1085/jgp.60.1.102
复制
发表时间：
1972-01-01
影响因子：
3.8
通讯作者：
ARCH, S
中科院分区：
文献类型：
--
作者：
ARCH, S
本文研究了加州瓢虫囊细胞神经元产卵激素的生物合成。将袋细胞与亮氨酸-3H在体外孵育30分钟，并在追逐培养基中冲洗不同的时间段。通过在十二烷基硫酸钠聚丙烯酰胺凝胶上电泳匀浆来测定掺入的标记在细胞内蛋白质之间的分布。冲洗时间短于30分钟的结果表明，主要的合成产物是25，000道尔顿蛋白质。随着冲洗时间的延长，这种物质减少，两种较低分子量的物质变得突出。这种放射性的重新分布是定量的，并且在冲洗期间不会受到蛋白质合成抑制的阻碍。温度降低10 ℃（从15 ℃）阻止了再分配。这些数据被解释为表明25，000道尔顿分子是酶促裂解产生两种较低分子量产物的前体。一种产物是12，000道尔顿的分子，它保留在细胞体中。另一种是分子量小于10，000道尔顿的分子，从胞体输出到结缔组织的神经血管区。用高[K+]培养基灌注这些区域导致该产品释放到培养基中。由此推断，该产物为6000道尔顿的产蛋激素（ELH）。
Biosynthesis of the egg-laying hormone in the bag cell neurons of Aplysia californica was studied. Bag cells were incubated with leucine-3H in vitro for 30 min and rinsed for variable periods of time in a chase medium. The distribution of incorporated label among proteins within the cells was assayed by electrophoresis of an homogenate on sodium dodecyl sulfate polyacrylamide gels. Results from rinse times shorter than 30 min revealed that the predominant synthetic product is a 25,000 dalton protein. With longer rinse times, this species was reduced and two species of lower molecular weight became prominent. This redistribution of radioactivity was quantitative and was not prevented by inhibition of protein synthesis during the rinse. A 10 0C reduction in temperature (from 15 C) blocked the redistribution. These data are interpreted to indicate that the 25,000 dalton molecule is a precursor which is cleaved enzymatically to yield two lower molecular weight products. One product is a 12,000 dalton molecule which remains in the cell bodies. The other is a molecule of< 10,000 daltons which is exported from the somata into the neurohemal regions of the connective tissue. Perfusion of these regions with high [K+] medium results in the release of this product into the medium. It is concluded that this product is the 6000 dalton egg-laying hormone (ELH).