Tetraodon nigroviridis as a nonlethal model of infectious spleen and kidney necrosis virus (ISKNV) infection.
Tetraodon nigroviridis as a nonlethal model of infectious spleen and kidney necrosis virus (ISKNV) infection.
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DOI：
10.1016/j.virol.2010.07.003
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发表时间：
2010-10
期刊：
影响因子：
3.7
通讯作者：
Xiaopeng Xu;Lichao Huang;S. Weng;Jing Wang;Ting Lin;Junliang Tang;Zhongsheng Li;Qing-xia Lu;Qiong Xia;Xiaoqiang Yu;Jianguo He
中科院分区：
文献类型：
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作者：
Xiaopeng Xu;Lichao Huang;S. Weng;Jing Wang;Ting Lin;Junliang Tang;Zhongsheng Li;Qing-xia Lu;Qiong Xia;Xiaoqiang Yu;Jianguo He
传染性脾肾坏死病毒（ISKNV）是虹彩病毒科巨细胞病毒属的模式种。我们以前建立了一个高死亡率的斑马鱼ISKNV感染模型（Danio rerio）。本研究建立了一种非致死性的黑绿河豚ISKNV感染模型。ISKNV感染不会引起河豚的致死性疾病，但可感染该物种的几乎所有器官。电镜下可见ISKNV颗粒。免疫荧光和实时荧光定量PCR检测结果表明，感染后14 d，病毒粒子和感染细胞几乎全部被清除。银鱼和斑马鱼感染ISKNV后，IFN-γ和TNF-α基因的表达谱存在显著差异。非致死性感染ISKNV的Tetraodon动物模型的建立为巨细胞病毒病、鱼类免疫系统和病毒嗜性的研究提供了一种有价值的工具。
Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus, family Iridoviridae. We have previously established a high mortality ISKNV infection model of zebrafish (Danio rerio). In this study, a nonlethal Tetraodon nigroviridis model of ISKNV infection was established. ISKNV infection did not cause lethal disease in Tetraodon but could infect almost all the organs of this species. Electron microscopy showed ISKNV particles were present in infected tissues. Immunofluorescence and quantitative real-time PCR analysis showed that nearly all the virions and infected cells were cleared at 14d postinfection. The expression profiles of interferon-γ and tumor necrosis factor-α gene in response to ISKNV infection were significantly different in Tetraodon and zebrafish. The establishment of the nonlethal Tetraodon model of ISKNV infection can offer a valuable tool complementary to the zebrafish infection model for studying megalocytivirus disease, fish immune systems, and viral tropism.