Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
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DOI：
10.1016/j.chroma.2009.04.017
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发表时间：
2009-06-12
影响因子：
4.1
通讯作者：
Xu, Hong-Xi
中科院分区：
文献类型：
--
作者：
Zhou, Yan;Xu, Gang;Xu, Hong-Xi
优化并建立了超高效液相色谱（UHPLC）-光电二极管阵列检测器-四极杆飞行时间串联质谱（QTOF-MS/MS）联用技术，用于12种鼠尾草属植物甲醇提取物中重要二萜类化合物的定性定量分析。通过主成分分析确定了丹参属植物的分类特征。所有质子化分子和后续碎片离子的精确质量测量误差在3 ppm以内，为解释各类二萜类化合物的碎片化途径提供了更高质量的结构信息。就这样。在10分钟内分离了来自不同鼠尾草属物种的总共21种二萜类化合物，并通过与真实标准品和文献的比较明确地或暂时地鉴定。经验证，该UHPLC-QTOF-MS/MS方法具有灵敏度、精密度和准确度，检测限为3.0-16 ng/ml。日内和粘土间的总体变化小于3%。方法的回收率在96.2- 101.8%之间，相对标准偏差（RSD）小于3.0%。结果表明，鼠尾草属植物中二萜类化合物的定性和定量差异不仅对鼠尾草属植物的化学分类学研究有重要意义，而且对大量相似样品的标准化和鉴别也有重要意义。(C)2009爱思唯尔有限公司版权所有。
An on-line ultra-high-performance liquid chromatography (UHPLC) coupled with photodiode-array detection and quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) method has been optimized and established for the qualitative and quantitative analysis of the important diterpenoids in the methanol extracts of 12 Salvia species. Specific marker components were identified for the classification of the Salvia samples by principal component analysis. The accurate mass measurement within 3 ppm error for all the protonated molecules and Subsequent fragment ions offers higher quality structural information for interpretation of fragmentation pathways of various groups of diterpenoids. Thus. a total of 21 diterpenoids from different Salvio species were separated within 10 min, and were unequivocally or tentatively identified via comparisons with authentic standards and literature. This UHPLC-QTOF-MS/MS method was validated to be sensitive, precise and accurate with the limit of detections at 3.0-16 ng/ml. and the overall intra-day and the inter-clay variations less than 3%. The recovery of the method was in the range of 96.2-101.8%, with relative standard deviation (RSD) less than 3.0%. The results demonstrated that the qualitative and quantitative differences in diterpenoids were not only useful for chemotaxonomy in some Salvia species but also for the standardization and differentiation of large numbers of similar samples. (C) 2009 Elsevier B.V. All rights reserved.