Extra Virgin Olive Oil Phenols Suppress Migration and Invasion of T24 Human Bladder Cancer Cells Through Modulation of Matrix Metalloproteinase-2
Extra Virgin Olive Oil Phenols Suppress Migration and Invasion of T24 Human Bladder Cancer Cells Through Modulation of Matrix Metalloproteinase-2
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DOI：
10.1080/01635581.2014.922204
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Lendaro, Eugenio
中科院分区：
文献类型：
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作者：
Coccia, Andrea;Bastianelli, Daniela;Lendaro, Eugenio
食用特级初榨橄榄油 (EVOO) 是地中海人的常见饮食习惯，似乎与某些类型的癌症（包括膀胱肿瘤）的发病率较低有关。 Metastases are the major cause of bladder cancer-related deaths and targeting cell motility has been proposed as a therapeutic strategy to prevent cancer spread.本研究旨在探讨从 EVOO 中提取的总酚对人移行性膀胱癌细胞系 T24 的潜在抗转移作用。我们还旨在验证 EVOO 提取物对肿瘤细胞发挥细胞毒作用，而不影响正常尿路上皮成纤维细胞。我们的结果表明，EVOO 提取物能够以剂量依赖的方式显着抑制 T24 膀胱细胞的增殖和运动。在相同的实验条件下，成纤维细胞的增殖和运动没有显着改变。此外，MMP-2 的酶活性仅在 T24 细胞中在无毒的 EVOO 提取物剂量下受到抑制。 qRT-PCR 显示 MMP-2 表达减少，同时金属蛋白酶组织抑制剂表达增加。 Our results may support the epidemiological evidences that link olive oil consumption to health benefits and may represent a starting point for the development of new anticancer strategies.
The consumption of extra virgin olive oil (EVOO), a common dietary habit of the Mediterranean people, seems to be related to a lower incidence of certain types of cancer including bladder neoplasm. Metastases are the major cause of bladder cancer-related deaths and targeting cell motility has been proposed as a therapeutic strategy to prevent cancer spread. This study aimed to investigate the potential antimetastatic effect of total phenols extracted from EVOO against the human transitional bladder carcinoma cell line T24. We also aimed at verifying that EVOO extract exerts cytotoxic effect on tumor cells without affecting normal urothelial fibroblasts. Our results show that EVOO extract can significantly inhibit the proliferation andmotility of T24 bladder cells in a dose-dependent manner. In the same experimental conditions fibroblast proliferation andmotility were not significantly modified. Furthermore the enzymatic activity of MMP-2 was inhibited at nontoxic EVOO extract doses only in T24 cells. The qRT-PCR revealed a decrease of the MMP-2 expression and a simultaneous increase of the tissue inhibitors of metalloproteinases expression. Our results may support the epidemiological evidences that link olive oil consumption to health benefits and may represent a starting point for the development of new anticancer strategies.