Magnetic resonance imaging and immunoblot analyses in rats with experimentally induced cerebral alveolar echinococcosis.
Magnetic resonance imaging and immunoblot analyses in rats with experimentally induced cerebral alveolar echinococcosis.
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发表时间：
2003-12
影响因子：
0.8
通讯作者：
T. Asanuma;Yukari Matsumoto;M. Takiguchi;O. Inanami;M. Nakao;K. Nakaya;A. Ito;A. Hashimoto;M. Kuwabara
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文献类型：
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作者：
T. Asanuma;Yukari Matsumoto;M. Takiguchi;O. Inanami;M. Nakao;K. Nakaya;A. Ito;A. Hashimoto;M. Kuwabara
应用核磁共振成像(MRI)和免疫印迹(Western印迹)技术，对实验性大鼠继发性脑泡状棘球虫病(AE)的早期阶段进行了研究。36只雌性Wistar大鼠(6~8周龄)脑内注射10%的包虫病幼虫组织匀浆，其中微泡和原头节的浓度分别为3.8和1.5×10(4)/ml。为了观察大鼠脑的精细结构，在7.05T强磁场下进行了MRI检查，并进行了组织学检查。在注射匀浆两周后，T2加权磁共振图像显示大脑皮层有一个高信号区域。注射后三周，根据组织学检查结果，发现该区域有囊性病变。在注射后6周和13周，在T2加权和质子密度磁共振图像上，分别观察到与角质层增生和随后的钙化相对应的信号空白区。另一方面，在注射后9周，用多房棘球绦虫两个表位(Em18和Em16)的抗体对血清进行免疫印迹分析，可识别出AE。利用本实验建立的继发性脑AE动物模型，认为MRI方法适用于继发性脑AE的早期诊断。
The early stage of experimentally induced secondary cerebral alveolar echinococcosis (AE) in rats was investigated by use of magnetic resonance imaging (MRI) and immunoblot (western blot) analyses. Thirty-six female Wistar rats (6 to 8 weeks old) were injected intracranially with a 10% homogenate of echinococcal larval tissues in which the concentrations of microvesicles and protoscolices were estimated to be 3.8 and 1.5 x 10(4)/ml, respectively. To observe the fine structure of the rat brain, MRI was performed under a high magnetic field of 7.05 T. Histologic examination also was performed. The T2-weighted MR images revealed a hyperintense region in the cerebral cortex at two weeks after injection of the homogenate. At three weeks after injection, this region was found to have cysts on the basis of results of histologic examination. Signal-void regions corresponding to hyperplasia and the subsequent calcification of the cuticle layer at six and 13 weeks after injection, respectively, were observed in T2-weighted and proton density MR images. On the other hand, at nine weeks after injection, AE was discernible by use of western blot analysis of sera with antibodies of two epitopes (Em18 and Em16) of E. multilocularis. Using this secondary cerebral AE animal model, it was concluded that the MRI method was suitable for early detection of secondary cerebral AE.