Effects of apolipoprotein E genotypes and other risk factors on the development of coronary artery disease in Southern Turkey
Effects of apolipoprotein E genotypes and other risk factors on the development of coronary artery disease in Southern Turkey
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DOI：
10.1016/s0009-8981(01)00624-6
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发表时间：
2001-10-01
影响因子：
5
通讯作者：
Kayrin, L
中科院分区：
文献类型：
--
作者：
Attila, G;Acartürk, E;Kayrin, L
背景：载脂蛋白E（apoE）在脂蛋白代谢和脂质转运中起重要作用.载脂蛋白E基因型与冠状动脉疾病（CAD）和其他危险因素之间的关系已被许多研究者描述。本研究的目的是探讨载脂蛋白E基因多态性和其他危险因素在冠心病的发展中的作用，在受试者的冠状动脉血管造影评价的手段。方法：研究人群包括199名受试者（114名男性和55名女性）。其中，107人患有CAD。用聚合酶链反应（PCR）扩增apoE基因，然后用CfoI限制性内切酶酶切。同时测定所有受试者的血脂水平和其他危险因素。结果：冠心病（+）组ε 2和ε 4等位基因频率及携带ε 4等位基因的基因型明显高于对照组（P < 0. 05）。冠心病（+）组除甘油三酯外，血脂均升高。发现apoE基因型、高血压、糖尿病、男性、年龄和吸烟是冠心病的独立预测因素。apoE等位基因与血脂无相关性。结论：我们得出结论，apoE多态性（e4等位基因的存在）与土耳其南部CAD的发展。在我们的研究中，我们没有观察到apoE等位基因对血脂水平的任何影响。(C)2001 Elsevier Science B. V.保留所有权利。
Background: Apolipoprotein E (apoE) plays a major role in lipoprotein metabolism and lipid transport. Associations between apoE genotypes, coronary artery disease (CAD) and other risk factors have been described by many investigators. The aim of this study was to investigate the role of apoE gene polymorphism and other risk factors in the development of CAD in subjects whose coronary arteries were evaluated by means of coronary angiography. Methods: The study population consisted of 199 subjects (114 male and 55 female). Of the total, 107 had CAD. The apoE gene was amplified by polymerase chain reaction (PCR) and then digested by CfoI restriction enzyme. The plasma lipid levels and other risk factors were also determined in all subjects. Results: The epsilon2 and epsilon4 allele frequencies and genotypes carrying epsilon4 allele were significantly higher in CAD (+) patients. Plasma lipids except triglycerides were increased in CAD (+) cases. We found that apoE genotypes, HT, DM, male gender, age and smoking were the independent predictors of CAD. There was no association between apoE alleles and lipids. Conclusion: We conclude that apoE polymorphism (presence of e4 allele) is associated with the development of CAD in Southern Turkey. In our study, we did not observe any effect of apoE alleles on lipid levels. (C) 2001 Elsevier Science B.V. All rights reserved.