Improved one-dimensional thin-layer chromatography of phospholipids in amniotic fluid.
Improved one-dimensional thin-layer chromatography of phospholipids in amniotic fluid.
复制标题
改进羊水中磷脂的一维薄层色谱法。
作者：
A. Pappas;R. Mullins;R. Gadsden
采用该提取程序和色谱法，六种羊水磷脂[磷脂酰胆碱（卵磷脂）、鞘磷脂、磷脂酰肌醇、磷脂酰丝氨酸、磷脂酰乙醇胺和磷脂酰甘油]在收到标本后不到70分钟内完全分离。移动的流动相（氯仿/石油醚/甲醇/乙酸，5/3/1.6/1，体积比）与市售7.5 × 10 cm硅胶板一起使用。将平板浸入醋酸铜/磷酸中，使磷脂可见。脂质体/鞘磷脂（L/S）比值通过光密度测定法测定。样品和标准品的Rf值重现性良好，L/S比也是如此，该方法简单且廉价。L/S比值（用这种方法测量的100份羊水标本）与胎儿状况之间的关系与其他报告一致。我们证实磷脂酰甘油是胎儿肺成熟度的敏感指标：当它在羊水中检测到时，新生儿总是没有呼吸窘迫综合征的症状。
With this extraction procedure and chromatographic method, six amniotic fluid phospholipids [phosphatidylcholine (lecithin), sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol] are completely resolved in less than 70 min from the time the specimen is received. The mobile phase (chloroform/petroleum ether/methanol/acetic acid, 5/3/1.6/1, by vol) is used with commercially available 7.5 X 10 cm silica-gel plates. The phospholipids are made visible by immersing the plate in cupric acetate/phosphoric acid. Lecithin/sphingomyelin (L/S) ratios are determined densitometrically. Rf values are well reproducible for both samples and standards, as are L/S ratios, and the method is simple and inexpensive. The relation between L/S ratios (for 100 specimens of amniotic fluid measured by this method) and fetal status is consistent with other reports. We confirm phosphatidylglycerol to be a sensitive indicator of fetal lung maturity: when it was detectable in amniotic fluid, the newborn invariably showed no symptoms of the respiratory distress syndrome.