Polysaccharide-polynucleotide Interaction (XI); Novel Separation System of RNAs by Using Schizophyllan-appended Column
Polysaccharide-polynucleotide Interaction (XI); Novel Separation System of RNAs by Using Schizophyllan-appended Column
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多糖-多核苷酸相互作用(XI)；
DOI：
10.1246/cl.2002.1240
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发表时间：
2002
影响因子：
1.6
通讯作者：
S. Shinkai
中科院分区：
文献类型：
--
作者：
Taro Kimura;Kazuya Koumoto;M. Mizu;K. Sakurai;S. Shinkai
裂叶植物属于β-1,3-葡聚糖家族，可以与特定的多核苷酸形成复合物我们通过构建裂叶植物附加柱来应用这种络合来特异性分离多核苷酸。色谱柱捕获了聚(C)和聚(A)，而对聚(G)没有任何亲和力，这与在均相溶液中得到的实验结果一致。结果表明，s-SPG色谱柱可作为一种新型的生物分子分离装置。
Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.