Chitosan/gelatin micro/nanofiber 3D composite scaffolds for regenerative medicine
Chitosan/gelatin micro/nanofiber 3D composite scaffolds for regenerative medicine
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DOI：
10.1080/15685543.2014.852016
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发表时间：
2014-05-04
影响因子：
2.6
通讯作者：
Hund, Rolf-Dieter
中科院分区：
文献类型：
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作者：
Hild, Martin;Toskas, Georgios;Hund, Rolf-Dieter
本文报道了用于再生医学的新型复合壳聚糖基针织三维纺织支架的制备。一个关键的研究目标仍然是制造具有确定尺寸和定向排列的纯壳聚糖超纤维纱线，这可能导致纺织结构，例如机织物和/或针织物。用具有足够抗拉强度的纯壳聚糖纤维构建了这种纺织结构。在微/纳米纤维复合材料的组合中，人们可以受益于微纤维的编织/针织结构所提供的强度和纺丝纳米纤维网的细胞播种能力。因此，用明胶纳米纤维对壳聚糖针织物进行功能化处理，为细胞播种提供了新的界面。纯壳聚糖和壳聚糖/明胶支架均被证明对成纤维细胞无细胞毒性。此外，通过体外成骨细胞培养实验，分析了功能化纺织支架的表面和界面对细胞长入和增殖的影响。
The present study reports on the fabrication of new composite chitosan-based knitted three-dimensional textile scaffolds for regenerative medicine. A critical research objective remained the fabrication of pure chitosan microfiber yarn with defined size and directional alignment, which could lead to textile structure, for example, woven and/or knitted fabrics. The construction of such textile structure with pure chitosan fibers possessing adequate tensile strength was recently realized in our laboratory. In the combination of micro/nanofiber composites, one can benefit from both the strength provided by the woven/knitted structure of the microfiber and the cell-seeding ability of the spun nanofiber mesh. Therefore, chitosan knitted fabrics were functionalized with gelatin nanofibers producing new interfaces for cell seeding. Both, pure chitosan and chitosan/gelatin scaffolds were proved non-cytotoxic to fibroblasts. Moreover, in vitro test with osteoblasts culture was carried out in order to analyze the influence of the surfaces and interfaces of the functionalized textile scaffold on cell ingrowth and proliferation.