Laser capture microdissection/GeneChip analysis of gene expression in glomerular cells in diabetic db/db mice
Laser capture microdissection/GeneChip analysis of gene expression in glomerular cells in diabetic db/db mice
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DOI：
10.1179/135100004225006786
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发表时间：
2004-01-01
期刊：
影响因子：
3.8
通讯作者：
Yoshikawa, T
中科院分区：
文献类型：
--
作者：
Naito, Y;Uchiyama, K;Yoshikawa, T
尽管在啮齿动物模型和人类中研究了糖尿病肾病发展过程中的基因表达模式，但仅表征了系膜或肾小球细胞中表达的小部分mRNA。在本研究中，我们报告了更大的组的成绩单显示显着的表达调制肾小球细胞从早期阶段的diabetic nephropathy.Methods：我们使用了12周龄的雌性db/db小鼠，啮齿类动物模型的2型糖尿病，和他们的非糖尿病db/m窝队友。通过激光捕获显微切割从小鼠肾脏获得肾小球细胞。cRNA的制备和靶标杂交根据Affyphase GeneChip Eukaryotic Small Sample Target Labeling Assay Protocol（Version II）进行。结果：通过db/m和db/db小鼠之间的比较，鉴定出649个随着糖尿病诱导而表达增加的探针和340个在糖尿病肾脏中表达减少的探针。虽然这些基因中的一些先前已被证明在糖尿病肾病中发挥重要作用，但其中绝大多数从未被证明在肾病的发展过程中受到调控。结论：尽管这些基因在糖尿病肾病中的确切参与仍有待澄清，
Although the gene expression patterns during the development of diabetic nephropathy have been studied in both rodent models and humans, only a small portion of the mRNAs expressed in the mesangium or in glomerular cells has been characterized. In the present study we report larger groups of transcripts displaying significant expression modulation in glomerular cells obtained from the early phase of diabetic nephropathy.Methods: We used 12-week-old female db/db mice, a rodent model of type 2 diabetes, and their nondiabetic db/m litter-mates. Glomerular cells were obtained from the kidneys of mice by laser capture microdissection. Preparation of cRNA and target hybridization were performed according to the Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Protocol (Version II). The gene expression profile was determined by the mouse Expression Set 430A GeneChip.Results: By comparison between db/m and db/db mice, 649 probes that increased in expression with the induction of diabetes and 340 probes that decreased in diabetic kidneys were identified. Although some of these genes have previously been shown to play an important role in diabetic nephropathy, the large majority of them have never been demonstrated to be regulated during the development of nephropathy.Conclusions: Although the precise involvement of these genes in diabetic nephropathy remains to be clarified,