Ancient and recent horizontal invasions of drosophilids by P elements
Ancient and recent horizontal invasions of drosophilids by P elements
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DOI：
10.1007/s002390010121
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发表时间：
2000-12-01
影响因子：
3.9
通讯作者：
Pinsker, W
中科院分区：
文献类型：
--
作者：
Haring, E;Hagemann, S;Pinsker, W
被命名为M型和O型的两个不同亚科的P元件被认为是通过外部来源的水平传播入侵了黑腹果蝇种群的寄主物种。与从其他物种中分离的P元件的序列比较表明，O型的水平入侵一定是最近的事件，而M型的入侵应该发生在更遥远的过去。为了追踪O型分子的系统发育历史，使用类型特异的聚合酶链式反应(PCR)引物筛选了更多的分类群，以确定O型和M型分子的存在。根据927个碱基(14个类群)的序列数据推断的系统发育表明，O型元素在果蝇的Saltan和Wilistoni组的谱系中经历了较长的规则垂直传递周期。然而，从D，Wilistoni群的一个物种开始，他们被水平地传递到其他谱系。在最近的一波水平传播浪潮中，3个不同属的物种受到来自D affinis谱系的O型元素的入侵：Scaptomyza、Lordiphosa和暗果蝇亚群的兄弟种D.biasciata/D.imaii。从这些分类群中分离出的O型分子几乎相同(序列差异
P elements of two different subfamilies designated as M- and O-type are thought to have invaded host species in the Drosophila obscura group via horizontal transmission from external sources. Sequence comparisons with P elements isolated from other species suggested that the horizontal invasion by the O-type must have been a rather recent event, whereas the M-type invasion should have occurred in the more distant past. To trace the phylogenetic history of O-type elements, additional taxa were screened for the presence of O- and M-type elements using type-specific PCR primers. The phylogeny deduced from the sequence data of a 927-bp section (14 taxa) indicate that O-type elements have undergone longer periods of regular vertical transmission in the lineages of the saltans and willistoni groups of Drosophila. However, starting from a species of the D, willistoni group they were transmitted horizontally into other lineages. First the lineage of the D. affinis subgroup was infected, and finally, in a more recent wave of horizontal spread, species of three different genera were invaded by O-type elements from the D. affinis lineage: Scaptomyza, Lordiphosa, and the sibling species D. bifasciata/D. imaii of the Drosophila obscura subgroup. The O-type elements isolated from these taxa are almost identical (sequence divergence