Genetically modified microorganism Spingomonas paucimobilis UT26 for simultaneously degradation of methyl-parathion and γ-hexachlorocyclohexane
Genetically modified microorganism Spingomonas paucimobilis UT26 for simultaneously degradation of methyl-parathion and γ-hexachlorocyclohexane
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DOI：
10.1007/s10646-014-1224-8
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发表时间：
2014-03
期刊：
影响因子：
2.7
通讯作者：
W. Lan;T. Lu;Zhi-feng Qin;Xiu Shi;Jin Wang;Yun Hu;Bin Chen;Yi-Hang Zhu;Zheng Liu
中科院分区：
文献类型：
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作者：
W. Lan;T. Lu;Zhi-feng Qin;Xiu Shi;Jin Wang;Yun Hu;Bin Chen;Yi-Hang Zhu;Zheng Liu
利用细菌对农药残留进行生物修复是治理环境污染的一种高效、环保的方法。本研究通过将对硫磷水解酶基因导入六六六(γ-γ)降解菌UT26中，构建了一株命名为UT26XEGM的转基因微生物。为了减少其潜在的基因逃逸到环境中的风险，为了满足公众对生物安全的关注，还设计了一个自杀系统，在特定信号激活GMM的生理功能之前，不会干扰GMM的性能。该系统设计了由Pm启动子和thexylS基因共同控制的致死基因gEf和ecoRIR组成的自杀盒电路。外源基因的插入不影响细胞活力和原始降解特性。新型超滤膜能够同时降解甲基对硫磷和γ-六氯环己烷。在实验室规模试验中，重组菌成功地应用于混合农药残留的生物修复，经3-甲基苯甲酸酯诱导后具有自毁活性。
Bioremediation of pesticide residues by bacteria is an efficient and environmentally friendly method to deal with environmental pollution. In this study, a genetically modified microorganism (GMM) named UT26XEGM was constructed by introducing a parathion hydrolase gene into an initially γ-hexachlorocyclohexane (γ-HCH) degrading bacteriumSpingomonas paucimobilisUT26. In order to reduce its potential risk of gene escaping into the environment for the public concern on biosafety, a suicide system was also designed that did not interfere with the performance of the GMM until its physiological function was activated by specific signal. The system was designed with circuiting suicide cassettes consisting of killing genesgefandecoRIRfromEscherichia colicontrolled by Pm promoter and thexylSgene. The cell viability and original degradation characteristics were not affected by the insertion of exogenous genes. The novel GMM was capable of degrading methyl-parathion and γ-HCH simultaneously. In laboratory scale testing, the recombinant bacteria were successfully applied to the bioremediation of mixed pesticide residues with the activity of self-destruction after 3-methylbenzoate induction.