Chromatographic ECD fingerprints combined with a chemometric method used for the identification of three light-coloured unifloral honeys
Chromatographic ECD fingerprints combined with a chemometric method used for the identification of three light-coloured unifloral honeys
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DOI：
10.1039/c5ay01063b
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Cao, Wei
中科院分区：
文献类型：
--
作者：
Zhao, Jing;Cheng, Ni;Cao, Wei
本工作完成了三种浅色单花蜂蜜的鉴定。采用高效液相色谱-电化学检测法（HPLC-ECD）获得了不同产地的药材指纹图谱。从建立的指纹图谱中，选择17个响应值较高的共有峰作为分类变量。所有40个蜂蜜样品（包括林登，野豌豆villosa罗斯和油菜蜂蜜）正确分类，根据其植物来源，使用化学计量学方法。此外，还测定了样品中酚酸类物质的含量和理化参数。以理化参数和酚类成分为变量，总的分类正确率分别达到97.5%和100%。结果表明，电子捕获色谱指纹图谱所获得的信息是有用的花的起源鉴定的三个浅色蜂蜜。
The identification of three light-coloured unifloral honeys was accomplished in this work. Chromatographic fingerprints were obtained through high performance liquid chromatography-electrochemical detection (HPLC-ECD). From the established chromatographic fingerprints, 17 common peaks with relatively high responses were selected as variables for classification. All 40 honey samples (including linden, Vicia villosa Roth and rape honeys) were correctly classified according to their botanical origin using a chemometric method. Additionally, several physicochemical parameters and the content of phenolic acids in the samples were determined. Using physicochemical parameters and phenolic profiles as variables, the overall correct classification rate reached 97.5% and 100%, respectively. The results indicated that the information obtained from the chromatographic ECD fingerprints was useful for the identification of the floral origin of the three light-coloured honeys.