4-HYDROXYTAMOXIFEN, AN ACTIVE METABOLITE OF TAMOXIFEN, DOES NOT ALTER THE RADIATION SENSITIVITY OF MCF-7 BREAST-CARCINOMA CELLS IRRADIATED IN-VITRO
4-HYDROXYTAMOXIFEN, AN ACTIVE METABOLITE OF TAMOXIFEN, DOES NOT ALTER THE RADIATION SENSITIVITY OF MCF-7 BREAST-CARCINOMA CELLS IRRADIATED IN-VITRO
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DOI：
10.1007/bf00666060
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发表时间：
1994-01-01
影响因子：
3.8
通讯作者：
MULCAHY, RT
中科院分区：
文献类型：
--
作者：
SARKARIA, JN;MILLER, EM;MULCAHY, RT
测定了他莫昔芬的强抗雌激素代谢产物4-羟基三苯氧胺(4OH-)对体外照射的MCF-7细胞放射敏感性的影响。在含有10(-10)M雌二醇(E(2))的无酚红(E(2))培养液中加或不加10(-7)M4OH-，绘制MCF-7细胞的辐射剂量反应曲线。照射后立即将细胞转移到含有10(-10)ME(2)的附加牛血清的培养液中，以刺激集落形成。10(-7)M4OH-可抑制雌二醇刺激的细胞增殖，但对辐射敏感性无明显影响(p>；0.3)。照射后不加E(2)继续孵育24小时同样不能改变4OH-处理的MCF-7细胞的放射敏感性。这些研究表明，抗雌激素4OH-的生长抑制浓度并不改变人乳腺癌细胞系的体外辐射敏感性。
The effect of 4-hydroxytamoxifen (4OH-TAM), the potent anti-estrogenic metabolite of tamoxifen, on the radiosensitivity of MCF-7 cells irradiated in vitro was determined. Radiation dose response curves were generated for MCF-7 cells maintained and irradiated in phenol red-free medium containing 10(-10) M estradiol (E(2)) with or without 10(-7) M 4OH-TAM. Immediately after irradiation cells were transferred to medium containing 10(-10) ME(2) supplemented with bovine serum to stimulate colony formation. Estradiol-stimulated cell proliferation was inhibited by 10(-7) M 4OH-TAM, but radiation sensitivity was not significantly altered (p > 0.3). Continued incubation in the absence of E(2) for an additional 24 hours after irradiation likewise failed to alter the radiosensitivity of 4OH-TAM-treated MCF-7 cells. These studies indicate that growth-inhibitory concentrations of the anti-estrogen 4OH-TAM do not modify the in vitro radiation sensitivity of this line of human breast carcinoma cells.