On-chip microfluidic transport and bio/chemical sensing based on electrochemical bubble formation
On-chip microfluidic transport and bio/chemical sensing based on electrochemical bubble formation
复制标题
DOI：
10.1016/j.snb.2006.10.028
复制
发表时间：
2007-05-21
影响因子：
8.4
通讯作者：
Suzuki, Hiroaki
中科院分区：
文献类型：
--
作者：
Satoh, Wataru;Shimizu, Yoshifumi;Suzuki, Hiroaki
芯片上的微流控传输仅使用集成的微流控组件，包括两个泵和四个阀；此外，通过在混合通道中扩散混合溶液，在芯片上进行光学生物传感。泵和阀的基本组件是三电极系统和聚二甲基硅氧烷（PDMS）隔膜，用于在微流通道中分离电解质溶液和样品溶液。对于泵来说，氢泡在硅衬底上各向异性蚀刻通孔壁上的铂黑色工作电极周围生长或收缩。气泡的体积变化引起工作电极上的PDMS膜片变形，并使流道中的溶液被调动。同样的原理也用于打开或关闭流道中的阀门。从两个注入口引入两种不同的溶液，并通过泵和阀的顺序操作通过流道输送。当流动停止时，我们可以在混合通道中混合溶液。为了证明微流体系统对微分析系统的适用性，从两个注射口注入含有酶的溶液和含有酶底物的溶液，混合，并允许在混合通道中发生反应。对酶促反应进行了荧光检测，并对过氧化氢和谷氨酸进行了定量分析。(C) 2006 Elsevier B.V.版权所有
On-chip microfluidic transport was achieved using only integrated microfluidic components, including two pumps and four valves; furthermore, optical biosensing was conducted on the chip following the mixing of solutions by diffusion in a mixing channel. The basic components for the pumps and valves were three-electrode systems and a polydimethylsiloxane (PDMS) diaphragm to separate the electrolyte solution from the sample solution in the microflow channel. For the pump, hydrogen bubbles were grown or shrunk around platinum black working electrodes on the walls of anisotropically etched through-holes in a silicon substrate. The volume change of the bubbles caused deformation of the PDMS diaphragm over the working electrode, and the solution in the flow channel was mobilized. The same principle was used to open or close the valves in the flow channels. Two different solutions were introduced from two injection ports and transported through the flow channels by sequential operation of the pumps and the valves. When the flows were stopped, we were able to mix the solutions in a mixing channel. To demonstrate the applicability of the microfluidic system to microanalysis systems, a solution containing an enzyme and another solution containing enzyme substrates were injected from two injection ports, mixed, and allowed to react in the mixing channel. Fluorometric detection of the progress of enzymatic reactions and quantitative analysis of hydrogen peroxide and L-glutamate were conducted. (C) 2006 Elsevier B.V. All rights reserved.