Features of Microsystems for Cultivation and Characterization of Stem Cells with the Aim of Regenerative Therapy.
Features of Microsystems for Cultivation and Characterization of Stem Cells with the Aim of Regenerative Therapy.
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DOI：
10.1155/2016/6023132
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发表时间：
2016
影响因子：
4.3
通讯作者：
Park JY
中科院分区：
文献类型：
--
作者：
Ahn K;Kim SH;Lee GH;Lee S;Heo YS;Park JY
干细胞具有无限的再生治疗潜力，这要归功于它们的有利能力，这是可分化为恢复和自我更新所需的细胞类型。与传统方法相比，微系统已被证明更有助于干细胞研究，这依赖于其模拟体内细胞环境的有利特性以及其他有利的特性，例如用于分析的最小样品消耗和多程序。各种各样的微系统被开发用于干细胞研究;然而，再生治疗是生物学家和生物工程师的最终目标之一，因此，微技术的再生治疗靶向应用应该得到更多的重视和吸引力。在这篇综述中，我们介绍了利用众所周知的微技术（微孔，微图案，微流体通道）的观点的物理原理，以及如何这些系统和原则已被适当地实施表征干细胞，并找到可能的再生疗法的干细胞研究。生物学家可以获得有关微系统原理的信息，并将其应用于解决当前挑战，工程师可以了解传统微系统的局限性，并为进一步开发实用的微系统找到新的机会。通过工程师和生物学家的良好结合，利用微技术的再生治疗靶向干细胞研究将为人类疾病找到更合适的治疗方法。
Stem cells have infinite potential for regenerative therapy thanks to their advantageous ability which is differentiable to requisite cell types for recovery and self-renewal. The microsystem has been proved to be more helpful to stem cell studies compared to the traditional methods, relying on its advantageous feature of mimicking in vivo cellular environments as well as other profitable features such as minimum sample consumption for analysis and multiprocedures. A wide variety of microsystems were developed for stem cell studies; however, regenerative therapy-targeted applications of microtechnology should be more emphasized and gain more attractions since the regenerative therapy is one of ultimate goals of biologists and bioengineers. In this review, we introduce stem cell researches harnessing well-known microtechniques (microwell, micropattern, and microfluidic channel) in view point of physical principles and how these systems and principles have been implemented appropriately for characterizing stem cells and finding possible regenerative therapies. Biologists may gain information on the principles of microsystems to apply them to find solutions for their current challenges, and engineers may understand limitations of the conventional microsystems and find new chances for further developing practical microsystems. Through the well combination of engineers and biologists, the regenerative therapy-targeted stem cell researches harnessing microtechnology will find better suitable treatments for human disorders.