Cohesin SMC1β is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination
Cohesin SMC1β is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination
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DOI：
10.1038/ncb1135
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发表时间：
2004-06-01
影响因子：
21.3
通讯作者：
Jessberger, R
中科院分区：
文献类型：
--
作者：
Revenkova, E;Eijpe, M;Jessberger, R
姐妹染色单体内聚确保有丝分裂和减数分裂中染色体的忠实分离（1-4）。内聚蛋白复合物的减数分裂特异性组分，包括最近描述的SMC1异构体SMC1 β(5)，被认为是减数分裂姐妹染色单体内聚和DNA重组所必需的。在这里，我们发现smc1beta缺陷小鼠无论性别都是不育的。雄性减数分裂在粗线中受阻；女性减数分裂非常容易出错，但一直持续到中期II。前期轴向元件（ae）明显缩短，染色质从ae进一步延伸，染色体突触不完整，染色体臂和着丝粒中的姐妹染色单体内聚过早丧失。此外，交叉相关的重组灶缺失或减少，减数分裂特异性核周端粒排列受损。因此，SMC1beta在减数分裂内聚、ae组装、突触、重组和染色体运动中起关键作用。
Sister chromatid cohesion ensures the faithful segregation of chromosomes in mitosis and in both meiotic divisions(1-4). Meiosis-specific components of the cohesin complex, including the recently described SMC1 isoform SMC1beta(5), were suggested to be required for meiotic sister chromatid cohesion and DNA recombination. Here we show that SMC1beta-deficient mice of both sexes are sterile. Male meiosis is blocked in pachytene; female meiosis is highly error-prone but continues until metaphase II. Prophase axial elements (AEs) are markedly shortened, chromatin extends further from the AEs, chromosome synapsis is incomplete, and sister chromatid cohesion in chromosome arms and at centromeres is lost prematurely. In addition, crossover-associated recombination foci are absent or reduced, and meiosis-specific perinuclear telomere arrangements are impaired. Thus, SMC1beta has a key role in meiotic cohesion, the assembly of AEs, synapsis, recombination, and chromosome movements.