tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene
tk Enzyme expression in differentiating muscle cells is regulated through an internal segment of the cellular tk gene
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tk 分化肌肉细胞中的酶表达通过细胞 tk 基因的内部片段进行调节
DOI：
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发表时间：
1984
影响因子：
5.3
通讯作者：
S. McKnight
中科院分区：
文献类型：
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作者：
G. Merrill;S. Hauschka;S. McKnight
当培养的骨骼肌细胞终末分化时，胸苷激酶(Tk)酶的表达被关闭。这种调节是由细胞tk mRNA丰度的快速和特异性下降所介导的。同时利用鸡细胞tk基因和疱疹病毒tk基因将tk基因缺陷的小鼠成肌细胞转化为tk阳性表型。转化细胞tk基因的成肌细胞在终末分化时有效地调节tk酶的活性。相反，转化了疱疹病毒tk基因的成肌细胞在复制后的肌肉细胞中继续表达tk酶活性。当细胞tk基因的启动子被疱疹病毒tk基因的启动子取代时，保留了一种受调节的表达模式。此外，当细胞tk基因的3‘端被去除并被病毒tk基因的末端取代时，细胞tk基因在肌肉末端分化过程中受到适当的调节。因此，足以确定其调控的细胞tk基因元件完全是基因内的。
Thymidine kinase (tk) enzyme expression is shut down when cultured skeletal muscle cells terminally differentiate. This regulation is mediated by a rapid and specific decline in the abundance of cellular tk mRNA. tk-deficient mouse myoblasts were transformed to the tk-positive phenotype by using both the cellular tk gene of the chicken and the herpesvirus tk gene. Myoblasts transformed with the cellular tk gene effectively regulate tk enzyme activity upon terminal differentiation. Conversely, myoblasts transformed with the herpesvirus tk gene continue to express tk enzyme activity in postreplicative muscle cells. A regulated pattern of expression is retained when the promoter of the cellular tk gene is replaced by the promoter of the herpesvirus tk gene. Moreover, the cellular tk gene is appropriately regulated during terminal muscle differentiation when its 3' terminus is removed and replaced by the terminus of the viral tk gene. Thus, the element of the cellular tk gene sufficient to specify its regulation is entirely intragenic.