Changing localizations of site-specific sperm surface antigens during sea urchin fertilization.
Changing localizations of site-specific sperm surface antigens during sea urchin fertilization.
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在海胆受精过程中改变位点特异性精子表面抗原的定位。
DOI：
10.1002/jez.1402510109
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Ward,RD
中科院分区：
文献类型：
--
作者：
Nishioka,D;Gorman,EA;Ward,RD
间接免疫荧光研究表明，单抗J18/2可与紫球虫成熟精子顶体和尾部的表面抗原特异性结合。在暴露于蛋冻或离子载体A23187引发顶体反应后的5分钟内，这些表面抗原就可以在头部的外侧区域被检测到，因此精子的整个表面都被标记了。多精受精。紫癜卵在受精后不同时间固定并暴露于单抗J18/2后发现，这些表面抗原很快被结合到卵质膜上，最早在受精后1.5min开始分散。在随后的时间里，它们会进一步分散，这样在45分钟时，它们就会分布在整个卵子表面。这些结果表明，单抗J18/2识别的精子表面成分在顶体反应过程中获得了横向分散的能力，并在受精后继续在卵质膜中分散。
Indirect immunofluorescence studies show that monoclonal antibody (mAb) J18/2 binds site‐specifically to surface antigens localized over the acrosome and tail regions of matureStrongylocentrotus purpuratusspermatozoa. Within 5 min after induction of the acrosome reaction by exposure to egg jelly or ionophore A23187, these surface antigens become detectable over the lateral region of the head so that the entire surface of the spermatozoon is labeled. Polyspermically fertilizedS. purpuratuseggs fixed at varying times after insemination and exposed to mAb J18/2 reveal that these surface antigens are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times, they undergo further dispersal so that by 45 min they are distributed over the entire surface of the egg. These results suggest that the sperm surface components recognized by mAb J18/2 gain the ability to disperse laterally during the acrosome reaction and proceed to do so in the egg plasma membrane after fertilization.