Validation of a capillary electrophoresis method for determination of 5-aminolevulinic acid and degradation products.
Validation of a capillary electrophoresis method for determination of 5-aminolevulinic acid and degradation products.
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验证用于测定 5-氨基乙酰丙酸和降解产物的毛细管电泳方法。
DOI：
10.1016/s0021-9673(00)00334-4
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
B. Gander
中科院分区：
文献类型：
--
作者：
A. Bunke;H. Schmid;G. Burmeister;H. Merkle;B. Gander
建立了毛细管电泳法同时测定水溶液中5-氨基乙酰丙酸(ALA)及其降解产物2，5-二羧乙基-3，6-二氢吡嗪和2，5-二羧乙基吡嗪的方法。对优化后的方法进行了专属性、精密度、线性、检出限、定量限、稳健性等方面的验证。根据ALA峰对降解产物进行了定量。采用胶束电动色谱法，在运行缓冲液中加入十二烷基硫酸钠，直接进样含丙氨酸的水包油乳状液，即无需样品前处理。
A capillary electrophoresis method was developed for simultaneous quantification of 5-aminolevulinic acid (ALA) and its degradation products 2,5-dicarboxyethyl-3,6-dihydropyrazine and 2,5-dicarboxyethylpyrazine in aqueous solution within a total analysis time of 9 min. The optimized method was validated with respect to specificity, precision, linearity, limits of detection and quantitation, and robustness. The degradation products were quantified with respect to the ALA peak. A related micellar electrokinetic chromatography method, involving the addition of sodium dodecylsulfate to the running buffer solution, was applied for direct injection of an oil–in–water emulsion containing ALA, i.e. without sample pretreatment.