The Preventive Effect of Oxytocin on Retinopathy in Streptozotocin-Induced Diabetic Rats
The Preventive Effect of Oxytocin on Retinopathy in Streptozotocin-Induced Diabetic Rats
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DOI：
10.4274/tjo.galenos.2018.47897
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发表时间：
2019-04-01
影响因子：
1.1
通讯作者：
Taskiran, Dilek
中科院分区：
文献类型：
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作者：
Degirmenci, Cumali;Afrashi, Filiz;Taskiran, Dilek
目的：本研究的目的是探讨玻璃体内和腹腔内使用催产素（OT）对糖尿病大鼠视网膜病变的影响。材料和方法：24只6-8周龄的成年雄性和雌性Sprague道利大鼠用于本研究。通过腹腔注射链脲佐菌素诱导大鼠糖尿病。48小时后，通过测量糖尿病大鼠的血糖水平为260 mg/dl（14.4 mmol/L）或更高来验证糖尿病。将大鼠分为4组，分别为：玻璃体内注射生理盐水组（0.01 mL生理盐水每周一次）、玻璃体内注射OT组（10 μ U/μ L OT每周一次）、腹腔注射生理盐水组（1 mL/d）和腹腔注射OT组（100 IU/kg OT/d）。装有27号针头的汉密尔顿注射器用于腹膜内注射，而31号针头用于玻璃体内注射。治疗4周后，将大鼠安乐死，以评估外核层（ONL）的厚度，血管内皮生长因子（VEGF）的免疫表达，和血浆VEGF水平从血液样本获得的心脏puncture.Results：视网膜横截面的形态测量分析表明，玻璃体内和腹腔内OT显着增加ONL厚度相比，生理盐水治疗组。与生理盐水组相比，OT处理显著降低VEGF蛋白表达。血浆VEGF水平显着较高的生理盐水治疗组相比，OT treatment group.Conclusion：OT减少糖尿病视网膜病变的进展，特别是当玻璃体内给药。据我们所知，这是首次尝试调查OT对糖尿病视网膜病变的影响，并可能为进一步研究提供新的领域。
Objectives: The aim of this study was to investigate the impact of intravitreal and intraperitoneal use of oxytocin (OT) on retinopathy in streptozotocin-induced diabetic rats.Materials and Methods: Twenty-four 6-8-week-old adult male and female Sprague Dawley rats were used in the study. Diabetes was induced in the rats with a single injection of intraperitoneal streptozotocin. Diabetes was verified after 48 hours by measuring blood glucose levels of 260 mg/dl (14.4 mmol/L) or higher in diabetic rats. The rats were divided into 4 groups and treated as follows: intravitreal physiological saline group (0.01 mL saline weekly), intravitreal OT group (10 mu U/mu L OT weekly), intraperitoneal physiological saline group (1 mL daily), and intraperitoneal OT group (100 IU/kg OT daily). Hamilton syringes fitted with 27-gauge needles were used for intraperitoneal injections while 31-gauge needles were used for intravitreal injection. After 4 weeks of treatment the rats were euthanized to evaluate outer nuclear layer (ONL) thickness, vascular endothelial growth factor (VEGF) immunoexpression, and plasma VEGF levels from blood samples obtained by cardiac puncture.Results: Morphometric analysis of retinal cross-sections showed that intravitreal and intraperitoneal OT significantly increased ONL thickness compared to physiological saline-treated groups. Also, OT treatment significantly decreased VEGF protein expression compared with the physiological saline groups. Plasma VEGF level was significantly higher in the physiological saline treatment group compared to the OT treatment group.Conclusion: OT reduces diabetic retinopathy progression, particularly when administered intravitreally. To our knowledge, this is the first attempt to investigate the impact of OT on diabetic retinopathy and may provide a new area for further research.