Effect of ozone and open air factor against aerosolized Micrococcus luteus
Effect of ozone and open air factor against aerosolized Micrococcus luteus
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DOI：
10.4315/0362-028x-70.12.2769
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发表时间：
2007-12-01
影响因子：
2
通讯作者：
Griffith, Chris
中科院分区：
文献类型：
--
作者：
Bailey, Roger;Fielding, Louise;Griffith, Chris
由于对清洁失败、生物气溶胶的作用以及病原体的环境和临床持久性的关注增加，新型去污剂的评估变得越来越重要。在20世纪70年代确定了露天因子（OAF;高活性化学物质的集合）的杀菌特性;然而，人工生成的OAF的潜在实际应用直到最近才被考虑。研究了OAF对藤黄微球菌的抑制作用。通过将单萜以2.0（高）、0.75（中）或0.3（低）mgm（-3）h（-1）的浓度输送到臭氧化空气（0.1 ppm）中，在生物气溶胶试验室中产生和分布OAF。M.在2、5、10、20和60分钟后，对黄体进行雾化，并测定可培养存活菌的数量。通过在全玻璃撞击器流体中的需氧平板计数来对可培养细菌进行计数。使用单因素或双因素方差分析对数据进行统计学显著性分析。当气溶胶细菌暴露于臭氧单独（0.05，0.1，和2 ppm），一个显着的（高达3 log）减少观察到在所有浓度，效果是时间依赖性的。当暴露于单独的环状单萜时，测试样品和对照之间没有显著差异。当暴露于OAF（在0.1 ppm的臭氧高，中浓度）有显着差异后20分钟。这些减少显着大于单独使用臭氧在0.1 ppm。OAF是一种潜在的有效抗菌剂，可以减少空气中的微生物负荷。由于该技术使用环境中天然存在的反应化合物，因此对健康的风险可能低于与臭氧或其他气体处理相关的风险。然而，这一假设需要进一步调查。
Because of increased concerns over failures in cleaning, the role of bioaerosols, and the environmental and clinical persistence of pathogens, the evaluation of novel decontaminants is increasingly important. The bactericidal properties of open air factor (OAF; a collection of highly reactive chemical species) were identified in the 1970s; however, the potential practical applications of artificially generated OAF have been considered only recently. In this study, the effects of OAF against Micrococcus luteus were investigated. OAF was generated and distributed in a bioaerosol test chamber by delivery of monoterpenes into ozonated air (0.1 ppm) at concentrations of 2.0 (high), 0.75 (medium), or 0.3 (low) mgm(-3) h(-1). M. luteus was aerosolized, and the number of culturable survivors was determined after 2, 5, 10, 20, and 60 min. Culturable bacteria were enumerated by aerobic plate counts in all-glass impinger fluid. Data were analyzed for statistical significance using one- or two-way analyses of variance. When aerosolized bacteria were exposed to ozone alone (0.05, 0.1, and 2 ppm), a significant (up to 3-log) reduction was observed at all concentrations, and the effect was time dependent. When exposed to the cyclic monoterpene alone, there were no significant differences between test samples and controls. When exposed to OAF (high and medium concentrations in 0.1 ppm ozone) there were significant differences after 20 min. These reductions were significantly greater than those achieved with ozone alone at 0.1 ppm. OAF is potentially an effective antibacterial agent that can reduce the microbial load in air. Because the technology uses reaction compounds naturally found in the environment, risks to health may be lower than those associated with ozone or other gaseous treatments. However, this hypothesis needs further investigation.