Purification of Biliary Protein and Its Effect on Calcium Carbonate Mineralization
Purification of Biliary Protein and Its Effect on Calcium Carbonate Mineralization
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DOI：
10.1002/cjoc.200890190
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发表时间：
2008-06
影响因子：
5.4
通讯作者：
Chengli Yao
中科院分区：
文献类型：
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作者：
Chengli Yao
采用凝胶过滤法从猪胆汁中分离胆汁蛋白。用荧光光谱法测定Ca ~（2+）与蛋白质的相互作用。结果表明，胆汁蛋白与Ca ~（2+）之间存在较强的配位作用。采用扫描电子显微镜、傅里叶变换红外光谱和粉末X射线衍射对BP体系中的CaCO 3晶体进行了表征。对胆汁蛋白质溶液中CaCO 3的可能形成机理进行了讨论。
The biliary protein (BP) was isolated from pig bile by gel filtration. The interaction between Ca2+ and protein was measured by fluorescence spectra. The result showed that there was a strong coordination between biliary protein and Ca2+. The CaCO3 crystals obtained in systems with and without BP were characterized by scanning electron microscopy, Fourier transform infrared spectrography and powder X-ray diffractometry. The possible formation mechanism of CaCO3 in biliary protein solution was discussed.