Enhanced l-Serine Production from Glycerol by Integration with Thermodynamically Favorable d-Glycerate Oxidation
Enhanced l-Serine Production from Glycerol by Integration with Thermodynamically Favorable d-Glycerate Oxidation
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DOI：
10.1021/acssuschemeng.2c00040
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发表时间：
2022-02
影响因子：
8.4
通讯作者：
Shiting Guo;Xiaoxu Tan;Jie-min Zhu;Chuanjuan Lü;Chunyu Yang;Qian Wang-;Chao Gao;P. Xu;Cuiqing Ma
中科院分区：
文献类型：
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作者：
Shiting Guo;Xiaoxu Tan;Jie-min Zhu;Chuanjuan Lü;Chunyu Yang;Qian Wang-;Chao Gao;P. Xu;Cuiqing Ma
l-Serine is an important platform chemical with wide applications. Fermentative synthesis ofl-serine suffers from cytotoxicity and undesired degradation ofl-serine. Here, we designed anin vitroenzymatic cascade forl-serine production from glycerol, an unavoidable byproduct of the biofuel industry. NAD+-independentd-lactate dehydrogenase fromEscherichia coliMG1655 (EcDLD) was found by screening to have high oxidase activity towardd-glycerate. This enzyme was thus implemented into the enzymatic cascade to accomplish the generation of hydroxypyruvate fromd-glycerate, the key step inl-serine production. After a simple concentration optimization of biocatalysts including alditol oxidase, EcDLD,l-alanine dehydrogenase, formate dehydrogenase, and catalase, 17.58 mMl-serine can be produced from 18.51 mM glycerol within 12 h. The productivity ofl-serine was 1.47 mM/h with a yield of 0.95 mol/mol. Besidesl-serine production, application of thein vitroenzymatic cascade with EcDLD might be expanded to other biochemical production from glycerol, further supporting the viability of biofuel industry.
