THE ASSOCIATION OF THIRST, SODIUM APPETITE AND VASOPRESSIN RELEASE WITH C-FOS EXPRESSION IN THE FOREBRAIN OF THE RAT AFTER INTRACEREBROVENTRICULAR INJECTION OF ANGIOTENSIN-II, ANGIOTENSIN-(1-7) OR CARBACHOL
THE ASSOCIATION OF THIRST, SODIUM APPETITE AND VASOPRESSIN RELEASE WITH C-FOS EXPRESSION IN THE FOREBRAIN OF THE RAT AFTER INTRACEREBROVENTRICULAR INJECTION OF ANGIOTENSIN-II, ANGIOTENSIN-(1-7) OR CARBACHOL
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DOI：
10.1016/0306-4522(95)00238-e
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发表时间：
1995-11-01
期刊：
影响因子：
3.3
通讯作者：
FITZSIMONS, JT
中科院分区：
文献类型：
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作者：
MAHON, JM;ALLEN, M;FITZSIMONS, JT
研究了血管紧张素II （0.1 nm）、血管紧张素-(1-7)（1 nm或100 nm）和萘醌（500 ng）对李斯特帽大鼠前脑c-fos表达的影响。在血管紧张素II和carbachol后，c-Fos蛋白在视前正中核、终板血管间质、皮质下器官、室旁核和视上核中呈强烈染色，血管紧张素II导致正中视前核和终板血管间质腹侧的c-Fos表达明显高于carbachol，而在室旁核和视上核中则相反。苯酚对这些区域的c-fos表达有更大的影响。然而，血管紧张素-(1-7)仅在终末板血管器官和正中视前核中诱导c-Fos蛋白，且这两个区域的细胞核染色数量和强度明显低于血管紧张素II或carbachol。各组Lister大鼠分别给予相同剂量的相同物质，但不包括较低剂量的血管紧张素-(1-7)，并测量60 min内水和1.8% NaCl的摄入量。血管紧张素II刺激了水和NaCl的摄入。对饮水量的影响几乎是立竿见影的（5分钟）。与血管紧张素II不同，Carbachol对NaCl摄入量（0.7 +/- 0.4 ml）影响不大。血管紧张素-(1-7)对水（1.1 +/- 0.3 ml）或NaCl （0.3 +/- 0.3 ml）摄入量没有影响。在体外循环注射相同剂量的这三种物质后，在其他各组大鼠中测量血管加压素的血浆水平，同样不包括较低剂量的血管紧张素-(1-7)。与注射脑脊液的大鼠相比，血管紧张素II和乙醇引起血浆加压素水平增加约5倍，但血管紧张素-(1-7)对加压素释放没有影响。因此，三种对口渴、钠食欲和抗利尿激素释放有不同影响的化合物诱导了前脑中不同的c-fos蛋白表达模式。以这种方式结合实验方法，就有可能确定参与某些行为和内分泌反应的大脑区域。
The effect of intracereboventricular injections of angiotensin II (0.1 nm), angiotensin-(1-7) (1 or 100 nm) and carbachol (500 ng) on c-fos expression was examined in the forebrain of Lister hooded rats. Intense staining of the c-Fos protein was found in the median preoptic nucleus, organum vasculosum of the lamina terminalis, subfornical organ, paraventricular nucleus, and supraoptic nucleus after angiotensin II and carbachol Angiotensin II caused significantly more c-fos expression in the ventral median preoptic nucleus and organum vasculosum of the lamina terminalis than carbachol, whereas in the paraventricular and supraoptic nuclei this was reversed, with carbachol having a greater effect on c-fos expression in these areas. Angiotensin-(1-7), however, only induced c-Fos protein in the organum vasculosum of the lamina terminalis and median preoptic nucleus with the number and the intensity of staining of the nuclei significantly less in both areas than after angiotensin II or carbachol. Separate groups of Lister rats were given i.c.v. injections of the same substances at the same doses, but excluding the lower dose of angiotensin-(1-7), and the intakes of water and 1.8% NaCl over 60 min were measured. Angiotensin II stimulated intakes of both water and NaCl. The effect on water intake was almost immediate ( 5 min). Carbachol, unlike angiotensin II, had little effect on NaCl intake (0.7 +/- 0.4 ml). Angiotensin-(1-7) had no effect on water (1.1 +/- 0.3 ml) or NaCl (0.3 +/- 0.3 ml) intakes. The plasma levels of vasopressin were measured after i.c.v. injection of the same three substances in the same doses, again excluding the lower dose of angiotensin-(1-7), in further groups of rats. Angiotensin II and carbachol caused an approximate five-fold increase in plasma vasopressin levels compared to cerebrospinal fluid-injected rats, but angiotensin-(1-7) had no effect on vasopressin release.Therefore, three compounds with widely differing effects on thirst, sodium appetite and vasopressin release induce distinctive patterns of c-fos protein expression in the forebrain. By combining experimental approaches in this way it is possible to determine areas of the brain which are involved in certain behavioural and endocrine responses.