Expression and interaction of small heat shock proteins (sHsps) in rice in response to heat stress
Expression and interaction of small heat shock proteins (sHsps) in rice in response to heat stress
复制标题
水稻中小热激蛋白（sHsps）响应热应激的表达和相互作用
DOI：
10.1016/j.bbapap.2014.02.010
复制
发表时间：
2014-04-01
影响因子：
3.2
通讯作者：
He, Huaqin
中科院分区：
文献类型：
--
作者：
Chen, Xinhai;Lin, Shoukai;He, Huaqin
水稻（Oryza sativa L）固有的不动性限制了它们避免热胁迫的能力，需要它们通过先天防御能力来应对热胁迫，而热休克蛋白在其中发挥了重要作用。在本研究中，在热胁迫下，日本裸在苗期和花期均上调了Hsp26.7、Hsp23.2、Hsp17.9A、Hsp17.4和Hsp16.9A的表达。随后，这5种sHsps在耐热水稻品种Co39中的表达量均显著高于热敏感水稻品种Azucena。这表明这5种sHsps的表达水平与水稻植株抵御热胁迫的能力呈正相关。因此，这5种sHsps的表达水平可作为筛选不同抗热胁迫能力水稻品种的生物标志物。通过nature - page分析，发现热胁迫下3个水稻品种的Hsp18.1、Hsp17.9A、Hsp17.7和Hsp16.9A参与一个蛋白复合物，并通过酵母2杂交进一步验证了Hsp18.1与Hsp17.7、Hsp18.1与Hsp17.9A、Hsp17.7与Hsp16.9A的相互作用。拉下实验也证实了热胁迫下水稻中Hsp17.7和Hsp16.9A的互作。综上所述，5种sHsps的上调是水稻耐热胁迫的关键步骤，之后一些sHsps组装成一个大的异聚寡聚复合物。此外，Hsp101通过蛋白-蛋白相互作用调控硫胺素的生物合成，Hsp82同源性影响氮代谢，而Hsp81-1参与热胁迫下水稻植株糖或淀粉合成的维持。这些结果为研究水稻sHsps的调控机制提供了新的思路。(C) 2014 Elsevier B.V.版权所有
The inherent immobility of rice (Oryza sativa L) limited their abilities to avoid heat stress and required them to contend with heat stress through innate defense abilities in which heat shock proteins played important roles. In this study, Hsp26.7, Hsp23.2, Hsp17.9A, Hsp17.4 and Hsp16.9A were up-regulated in Nipponbare during seedling and anthesis stages in response to heat stress. Subsequently, the expressing levels of these five sHsps in the heat-tolerant rice cultivar, Co39, were all significantly higher than that in the heat-susceptible rice cultivar, Azucena. This indicated that the expressive level of these five sHsps was positively related to the ability of rice plants to avoid heat stress. Thus, the expression level of these five sHsps can be regarded as bio-markers for screening rice cultivars with different abilities to avoid heat stress. Hsp18.1, Hsp17.9A, Hsp17.7 and Hsp16.9A, in the three rice cultivars under heat stress were found to be involved in one protein complex by Native-PAGE, and the interactions of Hsp18.1 and Hsp17.7, Hsp18.1 and Hsp17.9A, and Hsp17.7 and Hsp16.9A were further validated,by yeast 2-hybridization. Pull down assay also confirmed the interaction between Hsp17.7 and Hsp16.9A in rice under heat stress. In conclusion, the up-regulation of the 5 sHsps is a key step for rice to tolerate heat stress, after that some sHsps assembled into a large hetero-oligomeric complex. In addition, through protein-protein interaction, Hsp101 regulated thiamine biosynthesis, and Hsp82 homology affected nitrogen metabolism, while Hsp81-1 were involved in the maintenance of sugar or starch synthesis in rice plants under heat stress. These results provide new insight into the regulatory mechanism of sHsps in rice. (C) 2014 Elsevier B.V. All rights reserved.