Protection against respiratory syncytial virus (RSV) elicited in mice by plasmid DNA immunisation encoding a secreted RSV G protein-derived antigen
Protection against respiratory syncytial virus (RSV) elicited in mice by plasmid DNA immunisation encoding a secreted RSV G protein-derived antigen
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DOI：
10.1016/s0928-8244(00)00213-3
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发表时间：
2000-12-01
影响因子：
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通讯作者：
Power, UF
中科院分区：
文献类型：
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作者：
Andersson, C;Liljeström, P;Power, UF
构建了呼吸道合胞病毒(RSV)候选疫苗BBG2Na的两种不同变异体(胞质和分泌型)的表达载体，并对其进行了核酸免疫研究。利用Semliki森林病毒基因扩增系统的两种不同载体在体外细胞培养中适当地表达了BBG2Na。用该质粒载体免疫小鼠，只在接受BBG2Na分泌版本编码的小鼠中才能产生显著的血清抗BBG2Na抗体。与抗体诱导数据一致，消毒肺对RSV-A攻击的保护也只在这一组观察到。这些结果表明，抗原表达的靶向性(细胞内和分泌)将是设计核酸疫苗时要考虑的一个重要因素。(C)2000年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
Plasmid vectors encoding two different variants, one cytoplasmic and one secreted version, of a candidate vaccine BBG2Na to respiratory syncytial virus (RSV), were constructed and evaluated in a nucleic acid vaccination study. The two different vectors, which employed the Semliki Forest virus gene amplification system, were found to express BBG2Na appropriately in in vitro cell cultures. Immunisation of mice with the plasmid vectors elicited significant serum anti-BBG2Na IgG responses only in the mice receiving the plasmid encoding the secreted version of BBG2Na. Consistent with antibody induction data, sterilising lung protection against RSV-A challenge was also only observed in this group. These results indicate that the targeting of antigen expression (intracellular versus secreted) would be an important factor to consider in the design of nucleic acid vaccines. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.